Article

Probiotic Lactobacillus rhamnosus downregulates FCER1 and HRH4 expression in human mast cells.

Wihuri Research Institute, Helsinki 00140, Finland.
World Journal of Gastroenterology (impact factor: 2.47). 02/2011; 17(6):750-9. DOI:10.3748/wjg.v17.i6.750 pp.750-9
Source: PubMed

ABSTRACT To investigate the effects of four probiotic bacteria and their combination on human mast cell gene expression using microarray analysis.
Human peripheral-blood-derived mast cells were stimulated with Lactobacillus rhamnosus (L. rhamnosus) GG (LGG(®)), L. rhamnosus Lc705 (Lc705), Propionibacterium freudenreichii ssp. shermanii JS (PJS) and Bifidobacterium animalis ssp. lactis Bb12 (Bb12) and their combination for 3 or 24 h, and were subjected to global microarray analysis using an Affymetrix GeneChip(®) Human Genome U133 Plus 2.0 Array. The gene expression differences between unstimulated and bacteria-stimulated samples were further analyzed with GOrilla Gene Enrichment Analysis and Visualization Tool and MeV Multiexperiment Viewer-tool.
LGG and Lc705 were observed to suppress genes that encoded allergy-related high-affinity IgE receptor subunits α and γ (FCER1A and FCER1G, respectively) and histamine H4 receptor. LGG, Lc705 and the combination of four probiotics had the strongest effect on the expression of genes involved in mast cell immune system regulation, and on several genes that encoded proteins with a pro-inflammatory impact, such as interleukin (IL)-8 and tumour necrosis factor alpha. Also genes that encoded proteins with anti-inflammatory functions, such as IL-10, were upregulated.
Certain probiotic bacteria might diminish mast cell allergy-related activation by downregulation of the expression of high-affinity IgE and histamine receptor genes, and by inducing a pro-inflammatory response.

0 0
 · 
0 Bookmarks
 · 
58 Views
  • Source
    Article: Systemic effects of ingested Lactobacillus Rhamnosus: inhibition of mast cell membrane potassium (IKCa) current and degranulation.
    Paul Forsythe, Binxiang Wang, Ibrahim Khambati, Wolfgang A Kunze
    [show abstract] [hide abstract]
    ABSTRACT: Exposure of the intestine to certain strains lactobacillus can have systemic immune effects that include the attenuation of allergic responses. Despite the central role of mast cells in allergic disease little is known about the effect of lactobacilli on the function of these cells. To address this we assessed changes in rat mast cell activation following oral treatment with a strain of Lactobacillus known to attenuate allergic responses in animal models. Sprague Dawley rats were fed with L. rhamnosus JB-1 (1×10(9)) or vehicle control for 9 days. Mediator release from peritoneal mast cells (RPMC) was determined in response to a range of stimuli. Passive cutaneous anaphylaxis (PCA) was used to assess mast cell responses in vivo. The Ca(2+) activated K(+) channel (KCa3.1) current, identified as critical to mast cell degranulation, was monitored by whole cell patch-clamp. L. rhamnosus JB-1 treatment lead to significant inhibition of mast cell mediator release in response to a range of stimuli including IgE mediated activation. Furthermore, the PCA response was significantly reduced in treated rats. Patch-clamp studies revealed that RPMC from treated animals were much less responsive to the KCa3.1 opener, DCEBIO. These studies demonstrate that Ingestion of L. rhamnosus JB-1 leads to mast cell stabilization in rats and identify KCa3.1 as an immunomodulatory target for certain lactobacilli. Thus the systemic effects of certain candidate probiotics may include mast cell stabilization and such actions could contribute to the beneficial effect of these organisms in allergic and other inflammatory disorders.
    PLoS ONE 01/2012; 7(7):e41234. · 4.09 Impact Factor
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

Affymetrix GeneChip(®)
 
anti-inflammatory functions
 
Bifidobacterium animalis ssp
 
Certain probiotic bacteria
 
gene expression differences
 
genes
 
global microarray analysis
 
GOrilla Gene Enrichment Analysis
 
histamine H4 receptor
 
histamine receptor genes
 
human mast cell gene expression
 
L. rhamnosus
 
L. rhamnosus Lc705
 
Lactobacillus rhamnosus
 
MeV Multiexperiment Viewer-tool
 
microarray analysis
 
pro-inflammatory impact
 
pro-inflammatory response
 
probiotic bacteria
 
tumour necrosis factor alpha
 

Anna Oksaharju