Respiratory infection risk in athletes: association with antigen-stimulated IL-10 production and salivary IgA secretion. Scand J Med Sci Sports

School of Sport, Exercise and Health Sciences, Loughborough University, Leicestershire, UK.
Scandinavian Journal of Medicine and Science in Sports (Impact Factor: 2.9). 03/2011; 22(3):410-7. DOI: 10.1111/j.1600-0838.2010.01272.x
Source: PubMed


The purpose of this study was to examine factors influencing susceptibility to upper respiratory tract infections (URTI) in 18-35-year-old men and women engaged in endurance-based physical activity during the winter months. Eighty individuals (46 males, 34 females) provided resting blood and saliva samples for determination of markers of systemic immunity. Weekly training and illness logs were kept for the following 4 months. Thirty subjects did not experience an URTI episode and 24 subjects experienced 3 or more weeks of URTI symptoms. These illness-prone subjects had higher training loads and had ∼2.5-fold higher interleukin (IL)-4 and IL-10 production by antigen-stimulated whole blood culture than the illness-free subjects. Illness-prone subjects also had significantly lower saliva S-IgA secretion rate and higher plasma IgM (but not IgA or IgG) concentration than the illness-free subjects. There were no differences in circulating numbers of leukocyte subtypes or lymphocyte subsets between the illness-prone and illness-free subjects. The production of IL-10 was positively correlated and the S-IgA secretion rate was negatively correlated with the number of weeks with infection symptoms. It is concluded that high IL-10 production in response to antigen challenge and low S-IgA secretion are risk factors for development of URTI in physically active individuals.

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    Integrated Environmental Assessment and Management 03/2015; 11(4). DOI:10.1002/ieam.1636 · 1.38 Impact Factor
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    • "Aliquots were subsequently diluted with RPMI 1640 medium for use in the in vitro incubations. Stimulated whole blood and PBMC culture production of cytokines (IFN-í µí»¾, TNF-í µí»¼, IL-1í µí»½, IL-2, IL-4, IL-6, and IL-10) were determined as described previously [14]. Briefly, for the determination of baseline unstimulated cytokine production, 0.25 mL of heparinized whole blood or PBMC were added to 0.75 mL of RPMI 1640 medium and incubated at 37 "
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    ABSTRACT: Aim. Our aims were to determine the influence of plasma total 25-hydroxy vitamin D (25(OH)D) status on the plasma cytokine concentrations in athletes and the in vitro effects of different doses of 1, 25 dihydroxyvitamin D3 (1, 25(OH)2D3) on multiantigen stimulated cytokine production by whole blood and peripheral blood mononuclear cell (PBMC) cultures. Methods. Plasma samples from 43 athletes with high and low levels of 25(OH)D were assayed for the concentrations of cytokines. The whole blood samples and PBMCs from healthy subjects were incubated in vitro with a multi-antigen vaccine and different doses of added 1, 25(OH)2D3. The circulating cytokines and stimulated whole blood and PBMC culture production of cytokines were determined using a biochip assay. Results. The circulating interleukin-(IL-)10 and interferon-(IFN-) γ concentrations were significantly higher in the vitamin D sufficient athletes. Furthermore, the production of tumour necrosis factor-(TNF-) α, IL-6, IFN-γ, IL-2, and IL-10 by whole blood culture was significantly inhibited by 1, 25(OH)2D3 concentrations of 1000 pmol/L or 10000 pmol/L. Conclusions. We found that the influence of vitamin D on circulating cytokines might be different in athletes compared with nonathletes and cytokines production by whole blood culture was not influenced by 1, 25(OH)2D3 in concentrations within the normal healthy range.
    06/2014; 2014. DOI:10.1155/2014/820524
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    Mediators of Inflammation 06/2014; 2014(1):326803. DOI:10.1155/2014/326803 · 3.24 Impact Factor
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