Evaluation of fruit extracts of six Turkish Juniperus species for their antioxidant, anticholinesterase and antimicrobial activities. J Sci Food Agric

Department of Chemistry, Faculty of Arts and Sciences, Muğla University, 48121 Muğla, Turkey.
Journal of the Science of Food and Agriculture (Impact Factor: 1.71). 03/2011; 91(5):867-76. DOI: 10.1002/jsfa.4258
Source: PubMed


Juniperus L. (Cupressaceae) species are mostly spread out in the Northern Hemisphere of the world, and some of them are used as folkloric medicines. The fruits of some species are eaten. Since oxidative stress is one of the reasons for neurodegeneration and is associated with the Alzheimer's disease (AD), the extracts prepared from the fruits of six Juniperus species were screened for their antioxidant activity. Therefore, the extracts were also evaluated against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), which are chief enzymes in the pathogenesis of AD. In addition, antimicrobial activity was also evaluated.
In the β-carotene-linoleic acid assay, acetone extracts of J. oxycedrus subsp. oxycedrus, J. sabina and J. excelsa, and methanol extracts of J. phoenicea and J. sabina, effectively inhibited oxidation of linoleic acid. The hexane extracts of J. oxycedrus subsp. oxycedrus, J. foetidissima and J. phoenicea showed remarkable inhibitory effect against AChE and BChE.
Because of their high antioxidant activity, J. excelsa, J. oxycedrus subsp. oxycedrus, J. sabina and J. phoenicia might be used in the food industry as preservative agents or extension of the shelf-life of raw and processed foods. Since the hexane extracts of J. oxycedrus subsp. oxycedrus and J. foetidissima demonstrated significant anticholinesterase activity they should be considered as a potential source for anticholinesterase agents.

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Available from: Mehmet Öztürk, Jan 31, 2015
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    • "It is important, therefore, to determine their quantity and variety in different medicinal plants. Juniperus exclesa (Family-Cupressaceae) is used to treat respiratory tract diseases ranging from common cold to pneumonia, urinary tract inflammations, renal and gall bladder stones, and rheumatism (El Beyruthy, et al., 2008; Öztürk, et al., 2011; Sanchez de Medina, et al., 1994). "
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    ABSTRACT: The present study was designed to define the phenolic profile, flavonoid profile and the biological potentials of the ethanol extract of the berries of Juniperus excelsa growing in Lebanon. A total of 11 phenolic compounds, including gallic acid, vanilic acid, hydroxybenzoic acid, sinapic acid, ellagic acid, myrcetin, and hesperitin were identified and quantified in Juniperus excelsa using Reverse Phase High Performance Liquid Chromatography (RP-HPLC). The ethanolic extract of the berries of J. excelsa was examined for the antioxidant activity using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay. The extract showed a strong scavenging activity with an IC50 48.90μg/ml compared to the synthetic antioxidant butylatedhydroxyltoluene (BHT) (IC50 91.40μg/ml). This strong antioxidant activity can be attributed to the total phenolic content (17.95mg/g of extract) and the total flavonoidal content (3.817mg/g of extract) of the extract that were determined using the Folin-Ciocalteau (FC) and aluminum chloride methods, respectively.
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    • "Many biological activities have been reported for Juniperus sp. These include anti-inflammatory (Akkol, Guvenc, & Yesilada, 2009; Lesjak et al., 2011), diuretic, antiseptic (bacterial and fungal) (Cavaleiro, Pinto, Goncalves, & Salgueiro, 2006; Ennajar et al., 2009; Kour et al., 2008; Kusari, Lamshoft, & Spiteller, 2009; Miceli et al., 2009), anthelmintic (Kozan, Kupeli, & Yesilada, 2006), hypoglycaemic (Ju et al., 2008), hypotensive, abortifacient, antinociceptive (Akkol et al., 2009), antiviral (Sassi, Harzallah-Skhiri, Bourgougnon, & Aouni, 2008), anticancer (Kusari, Zuhlke, & Spiteller, 2010), antioxidant (Lesjak et al., 2011; Miceli et al., 2009; Orhan, Berkkan, Deliorman Orhan, Aslan, & Ergun, 2011; Orhan, Orhan, & Ergun, 2011; Ozturk, Tumen, Ugur, Aydogmus-Ozturk, & Topcu, 2011), anticholinesterase (Orhan, Orhan, et al., 2011; Ozturk et al., 2011) and analgesic properties (Moreno et al., 1998). They have also been found to be useful in the treatment of psoriasis (Koerfgen , 1964) and neurasthenic neurosis (Jonkov & Naidenov, 1974). "

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    • "Furthermore, J. oxycedrus ssp. oxycedrus and J. phoenicea were found to have high flavonoid and phenolic acid content which provide them to show remarkable antioxidant activity [5]. As in many of the diseases, antioxidant activity also helps to promote wound healing process [25]. "
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    ABSTRACT: Juniperus and Cupressus genera are mainly used as diuretic, stimulant, and antiseptic, for common cold and wound healing in Turkish folk medicine. In the present study, essential oils obtained from cones of Cupressus and berries of Juniperus were evaluated for their wound healing and anti-inflammatory effects. In vivo wound healing activity was evaluated by linear incision and circular excision experimental wound models, assessment of hydroxyproline content, and subsequently histopathological analysis. The healing potential was comparatively assessed with a reference ointment Madecassol. Additionally acetic-acid-induced capillary permeability test was used for the oils' anti-inflammatory activity. The essential oils of J. oxycedrus subsp. oxycedrus and J. phoenicea demonstrated the highest activities, while the rest of the species did not show any significant wound healing effect. The experimental study revealed that J. oxycedrus subsp. oxycedrus and J. phoenicea display remarkable wound healing and anti-inflammatory activities, which support the folkloric use of the plants.
    Evidence-based Complementary and Alternative Medicine 01/2012; 2012(7):728281. DOI:10.1155/2012/728281 · 1.88 Impact Factor
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