Article

Diagnostic potential of Stokes Shift spectroscopy of breast and prostate tissues-- a preliminary pilot study.

PG and Research Department of Physics, Jamal Mohamed College (Autonomous), Tiruchirappalli - 620020, Tamilnadu, India.
Technology in cancer research & treatment (Impact Factor: 1.94). 04/2011; 10(2):153-61.
Source: PubMed

ABSTRACT Stokes Shift (SS) Spectroscopy (SSS) of normal and abnormal breast and prostate tissues were studied. SS spectra is measured by simultaneously scanning both the excitation and emission wavelengths while keeping a fixed wavelength interval of Δλ = 20 nm. Characteristic, highly resolved peaks and significant spectral differences between normal and different pathological tissues of breast and prostate tissues were observed. The SS spectra of normal and different pathological breast and prostate tissues show the distinct peaks around 300, 350, 450, 500 and 600 nm may be attributed to tryptophan, collagen, NADH, flavin and porphyrin, respectively. Results of the current study demonstrate that the SS spectral changes due to tryptophan, collagen, hemoglobin, NADH, FAD and porphyrin have good diagnostic potential; therefore can be targeted as native tumor markers.

Full-text

Available from: Yang Pu, Jun 14, 2015
2 Followers
 · 
109 Views
  • Technology in cancer research & treatment 04/2011; 10(2):101. DOI:10.7785/tcrt.2012.500184 · 1.94 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: We discuss the use of time resolved fluorescence spectroscopy to extract fundamental kinetic information on molecular species in tissues. The temporal profiles reveal the lifetime and amplitudes associated with key active molecules distinguishing the local spectral environment of tissues. The femtosecond laser pulses at 310 nm excite the tissue. The emission profile at 340 nm from tryptophan is non-exponential due to the micro-environment. The slow and fast amplitudes and lifetimes of emission profiles reveal that cancer and normal states can be distinguished. Time resolved optical methods offer a new cancer diagnostic modality for the medical community.
    AIP Advances 03/2012; 2(1). DOI:10.1063/1.3697961 · 1.59 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The fluorophores of malignant human breast cells change their compositions that may be exposed in the fluorescence spectroscopy and blind source separation method. The content of the fluorophores mixture media such as tryptophan, collagen, elastin, NADH, and flavin were varied according to the cancer development. The native fluorescence spectra of these key fluorophores mixture media excited by the selective excitation wavelengths of 300 nm and 340 nm were analyzed using a blind source separation method: Nonnegative Matrix Factorization (NMF). The results show that the contribution from tryptophan, NADH and flavin to the fluorescence spectra of the mixture media is proportional to the content of each fluorophore. These data present a possibility that native fluorescence spectra decomposed by NMF can be used as potential native biomarkers for cancer detection evaluation of the cancer.
    SPIE photonic West 2014, San Francisco, California, USA; 02/2014