Estimate of Leaf Chlorophyll and Nitrogen Content in Asian Pear (Pyrus serotina Rehd.) by CCM-200

Notulae Scientia Biologicae 01/2011;
Source: DOAJ

ABSTRACT In many cases evaluation of chlorophyll and nitrogen content in plants need to destructive methods, more time and organic solvents. Application of chlorophyll meters save time and resources. The aim of this study was estimating of chlorophyll and nitrogen content in Asian pear leaves using non-destructive method and rapid quantification of chlorophyll by chlorophyll content meter (CCM-200). This study was conducted on 8 years old Asian pear trees during June 2008 in Tehran, Iran. To develop our regression model, the chlorophyll meter data were correlated with extracted chlorophyll and nitrogen content data obtained from DMSO and Kejeldal methods, respectively. The results showed that, there was positive and linear correlation between CCM-200 data and chlorophyll a (R²=0.7183), chlorophyll b (R²=0.8523), total chlorophyll (R²=0.90), and total nitrogen content (R²=0.76) in Asian pear leaves. Thus, it can be concluded that, CCM-200 can be used in order to predict both chlorophyll and nitrogen content in Asian pear leaves.

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    ABSTRACT: This study was conducted to determine effect of water stress on chlorophyll content and chlorophyll fluorescence parameter in young `Dezful’ olive trees. Three irrigation regimes (40% ETcrop, 65% ETcrop and 100% ETcrop) were used. After irrigation treatments were applied, some of biochemical parameters including chlorophyll a, b, total chlorophyll, chlorophyll fluorescence and also chlorophyll content index (C.C.I) were measured. Results of Analysis of variance showed that irrigation treatments had significant effect on chlorophyll a, total chlorophyll (chl a+b), C.C.I and Fv/Fm ratio. The amount of decreased chlorophyll a and total chlorophyll in plants were received 40% ETcrop were 51.55% and 46.86%, respectively, compared with 100% ETcrop.
    ICANRE 2012 : International Conference on Agricultural and Natural Resources Engineering; 09/2012
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    ABSTRACT: Chlorophyll fluorescence is a non-destructive tool for estimation of the efficiency of photochemistry and photosynthesis status in plants and has extensively been used in assessing plants response to environmental stresses. The aim of this study was to evaluate the effect of drought stress on chlorophyll fluorescence, chlorophyll content, chlorophyll index and biomass in four pistachio seedling rootstocks grown under greenhouse conditions. The rootstocks were Pistacia vera cv. Badami-e-Zarand, P. vera cv Ghazvini, P. vera cv. Sarakhs and P. mutica. The results showed that water stress reduced FV/Fm ratio ( which is an indicator for the maximum efficiency of photosystem II), chlorophyll contents and biomass in plants. Highest amount of FV/Fm ratio observed in control treatment (full irrigation) and had significant difference with severe stress. In the most cases there was no any significant difference among studied rootstocks except P. mutica that showed the highest quantum yield of PSII. The maximum and minimum chlorophyll concentrations in irrigation treatments also were obtained in control and severe stress, respectively. The effect of genotypes on the chlorophyll contents (a, b, total and CCI) were significant and the highest and the lowest of these parameters were belonged to P. mutica and P. vera cv. Sarakhs, respectively. In case of shoot, root and leaf dry weight, the rootstocks P. mutica and P. vera cv. Badami showed lower reduction under water stress. Based on the results, water stress had negative effects on pistachio seedling rootstocks and P. mutica showed more resistance than other genotypes.
    Journal of Water Research in Agriculture. 01/2014; 27(4):475-485.
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    Dataset: v68-196


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May 27, 2014