Article

Biodiesel production with microalgae as feedstock: from strains to biodiesel

Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, People's Republic of China.
Biotechnology Letters (Impact Factor: 1.74). 03/2011; 33(7):1269-84. DOI: 10.1007/s10529-011-0574-z
Source: PubMed

ABSTRACT Due to negative environmental influence and limited availability, petroleum-derived fuels need to be replaced by renewable biofuels. Biodiesel has attracted intensive attention as an important biofuel. Microalgae have numerous advantages for biodiesel production over many terrestrial plants. There are a series of consecutive processes for biodiesel production with microalgae as feedstock, including selection of adequate microalgal strains, mass culture, cell harvesting, oil extraction and transesterification. To reduce the overall production cost, technology development and process optimization are necessary. Genetic engineering also plays an important role in manipulating lipid biosynthesis in microalgae. Many approaches, such as sequestering carbon dioxide from industrial plants for the carbon source, using wastewater for the nutrient supply, and maximizing the values of by-products, have shown a potential for cost reduction. This review provides a brief overview of the process of biodiesel production with microalgae as feedstock. The methods associated with this process (e.g. lipid determination, mass culture, oil extraction) are also compared and discussed.

1 Follower
 · 
141 Views
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Nannochloropsis have attracted sustained interest from algal biodiesel researchers due to their high biomass accumulation rate and high lipid content. There are six recognized species in the Nannochloropsis genus that are phylogenetically divided into Nannochloropsis gaditana, Nannochloropsis salina, Nannochloropsis granulata, Nannochloropsis limnetica, Nannochloropsis oceanica and Nannochloropsis oculata. In this study, the potential of 9 Nannochloropsis species from the 6 genus for biodiesel production was evaluated by determining their growth rate, biomass accumulation, lipid productivity, lipid composition, fatty acid profiles and biodiesel properties. The results showed that the best strain was N. oceanica IMET1, with lipid productivity of 158.76±13.83mgL(-1)day(-1), TAG production of 1.67±0.20g/L, favorable fatty acid profiles of C16-C18 (56.62±1.96%) as well as suitable biodiesel properties of higher cetane number (54.61±0.25), lower iodine number (104.85±2.80gI2/100g) and relative low cloud point (3.45±0.50°C). N. oceanica IMET1 could be consider as valuable feedstock for microalgal biodiesel production.
    Bioresource Technology 06/2014; 167C:503-509. DOI:10.1016/j.biortech.2014.06.047 · 5.04 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The biodiesel production from photosynthetic microorganisms, is a topic of increasing research. However, the implications as for nutrient removal and lipid accumulation capacity are unknown, when grown in municipal wastewater with respect to be grown in a rich medium. In the present study the potential of two species of microalgae Chlorella vulgaris and Scenedesmus acutus and a cyanobacterium, Arthrospira maxima were comparatively evaluated to remove nutrients and accumulate useful lipids for producing biodiesel when growing into two wastewater qualities, raw (ARC ) and treated (ART), compared with a rich environment (AE) with commercial fertilizer. The cultures were performed in volumes of 1 L, with photoperiods of light:dark of 12 h:12 h. With the results an ANOVA was performed and treatment means were compared with Tukey's test (p≤0.05). It was obtained in cultures in ARC: 1) the higher biomass productivity (p≤0.05) for S. acutus (1.28 g L–1) and C. vulgaris (1.15 g L–1), whereas for A. maxima values were similar to ARC (0.93 g L–1) and AE (0.96 g L–1) (p >0.05). However, the highest rate of biomass increase corresponded to C. vulgaris (0.20 to 0.27 d–1) for the three culture media (p>0.001), whereas for S. acutus and A. maxima was between 0.06 and 0.14 d–1; 2) higher lipid accumulation (p≤0.05) (S. acutus, 28.3 %, C. vulgaris 22.4 % and A. maximum13.8 %); 3) the higher efficiency of nutrient removal, over 60 % of phosphorus and 90 % of organic nitrogen (p≤0.05) for all microorganisms; 4) higher production of biodiesel (between 109.4 and 244.6 mg L–1) for all species studied.
    Agrociencia 03/2014; 48(3):271-284. · 0.05 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Nannochloropsis have attracted sustained interest from algal biodiesel researchers due to their high biomass accumulation rate and high lipid content. There are six recognized species in the Nannochloropsis genus that are phylogenetically divided into Nannochloropsis gaditana, Nannochloropsis salina, Nannochloropsis granulata, Nannochloropsis limnetica, Nannochloropsis oceanica and Nannochloropsis oculata. In this study, the potential of 9 Nannochloropsis species from the 6 genus for biodiesel production was evaluated by determining their growth rate, biomass accumulation, lipid productivity, lipid composition, fatty acid profiles and biodiesel properties. The results showed that the best strain was N. oceanica IMET1, with lipid productivity of 158.76 ± 13.83 mg L−1 day−1, TAG production of 1.67 ± 0.20 g/L, favorable fatty acid profiles of C16–C18 (56.62 ± 1.96%) as well as suitable biodiesel properties of higher cetane number (54.61 ± 0.25), lower iodine number (104.85 ± 2.80 gI2/100 g) and relative low cloud point (3.45 ± 0.50 °C). N. oceanica IMET1 could be consider as valuable feedstock for microalgal biodiesel production.
    Bioresource Technology 01/2014; 167:503–509. · 5.04 Impact Factor