Article

Superior mineralization and neovascularization capacity of adult human metaphyseal periosteum-derived cells for skeletal tissue engineering applications.

Department of Orthopaedic Surgery, Shanghai Sixth People's Hospital, Shanghai Jiaotong University, 600 Yishan Road, Shanghai 200233, PR China.
International Journal of Molecular Medicine (impact factor: 1.98). 03/2011; 27(5):707-13. DOI:10.3892/ijmm.2011.634
Source: PubMed

ABSTRACT Bone tissue engineering is a promising cell-based strategy to treat bone defects. Mesenchymal stem cells from adult human bone marrow (hBMSCs) are a frequently used cellular source for bone tissue generation. However, the low frequency of these stem cells in adult bone marrow and their limited proliferation restrict their clinical utility. An alternative source of MSCs is the periosteum-derived cells, and these cells appear to be easy to harvest and expand ex vivo. We isolated human metaphyseal periosteum-derived cells (hMPCs) and hBMSCs from the same donors and compared their osteogenic capacity both in vitro and in vivo. After osteogenic induction in monolayer cultures, hMPCs resulted in more robust mineralization and expressed higher mRNA levels of BMP-2, osteopontin and osteocalcin than hBMSCs. Eight weeks after implantation of cellular-β-TCP scaffolds in immunodeficient mice, hMPC implantation showed higher neovascularization and higher percentage of mature bone formation than hBMSC implantation. In conclusion, hMPCs represent a promising cellular candidate for bone tissue engineering.

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    Article: Isolation of osteoprogenitors from human jaw periosteal cells: a comparison of two magnetic separation methods.
    Marcus Olbrich, Melanie Rieger, Siegmar Reinert, Dorothea Alexander
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    ABSTRACT: Human jaw periosteum tissue contains osteoprogenitors that have potential for tissue engineering applications in oral and maxillofacial surgeries. To isolate osteoprogenitor cells from heterogeneous cell populations, we used the specific mesenchymal stem cell antigen-1 (MSCA-1) antibody and compared two magnetic separation methods. We analyzed the obtained MSCA-1(+) and MSCA-1(-) fractions in terms of purity, yield of positive/negative cells and proliferative and mineralization potentials. The analysis of cell viability after separation revealed that the EasySep method yielded higher viability rates, whereas the flow cytometry results showed a higher purity for the MACS-separated cell fractions. The mineralization capacity of the osteogenic induced MSCA-1(+) cells compared with the MSCA-1(-) controls using MACS was 5-fold higher, whereas the same comparison after EasySep showed no significant differences between both fractions. By analyzing cell proliferation, we detected a significant difference between the proliferative potential of the osteogenic cells versus untreated cells after the MACS and EasySep separations. The differentiated cells after MACS separation adjusted their proliferative capacity, whereas the EasySep-separated cells failed to do so. The protein expression analysis showed small differences between the two separation methods. Our findings suggest that MACS is a more suitable separation method to isolate osteoprogenitors from the entire jaw periosteal cell population.
    PLoS ONE 01/2012; 7(10):e47176. · 4.09 Impact Factor
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Keywords

adult bone marrow
 
adult human bone marrow
 
Bone tissue engineering
 
bone tissue generation
 
clinical utility
 
hBMSC implantation
 
higher mRNA levels
 
higher neovascularization
 
higher percentage
 
hMPC implantation
 
human metaphyseal periosteum-derived cells
 
low frequency
 
mature bone formation
 
monolayer cultures
 
osteogenic capacity
 
osteogenic induction
 
periosteum-derived cells
 
promising cell-based strategy
 
stem cells
 
used cellular source
 

Daoyun Chen