Article

[Comparison of six molecular methods for differentiation of Lactococcus lactis subsp. lactis and cremoris].

Key Laboratory of Dairy Biotechnology and Bioengineering, Ministry of Education, Department of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China.
ACTA MICROBIOLOGICA SINICA 12/2010; 50(12):1670-6.
Source: PubMed

ABSTRACT To compare six molecular methods for differentiation of Lactococcus lactis subsp. lactis and cremoris.
Six molecular methods, such as 16S rRNA gene analysis, 16S-23S rRNA Intergenic spacer region polymorphism, Denaturing Gradient Gel Electrophoresis (DGGE), Random Amplified Polymorphic DNA (RAPD), Repetitive Extragenic Palindromic-PCR and Restricted Fragment Length Polymorphisms were used to differentiate the reference strains.
Except for 16S rRNA gene analysis and 16S-23S rRNA Intergenic spacer region polymorphism, the other protocols were competent.
The methods of DGGE and RAPD were more appropriate for differentiation of Lactococcus lactis subsp. lactis and cremoris.

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Keywords

16S rRNA gene analysis
 
16S-23S rRNA Intergenic spacer region polymorphism
 
Denaturing Gradient Gel Electrophoresis
 
differentiate
 
Lactococcus lactis subsp
 
Random Amplified Polymorphic DNA
 
reference strains
 
Repetitive Extragenic Palindromic-PCR
 
Restricted Fragment Length Polymorphisms