Role of Different Pre-Treatments on Composition and Rheology of Synovial Fluids

Polymers 01/2009; DOI: 10.3390/polym1010016
Source: DOAJ

ABSTRACT Different pre-treatments applied to synovial fluids (SF) before their analyses are tested to characterize SF after storage under different conditions and to investigate their evolution along a viscosupplementation treatment. The main techniques proposed involve steric exclusion chromatography with triple detection (SEC) and viscometry; it is the first time that such a study is developed. SEC gives the molecular weight distribution and concentration of hyaluronan (HA) and proteins separately; the steady state viscosity is always non-Newtonian and not directly related to SF composition. Pre-treatment of SF (storage in cold, filtration, centrifugation) allows us to conclude that, in order to store SF, it is best to freeze it, even if in some cases, viscosity is modified but not the composition. All the data obtained (including protease pre-treatment) allow us to conclude that a small fraction of HA-protein complex forms a loose 3D-network and controls the rheology.

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    ABSTRACT: Purpose Osteoarthritis (OA) is a degenerative joint disease that is characterized by the breakdown of articular cartilage. Rheology of synovial fluid is of interest because of its significance in joint lubrication. However, there are still many questions related to synovial fluid rheology and its relation with OA. Although viscosupplementation has been used as a treatment for OA for many years, its clinical effect remains controversial. Therefore, the purpose of this study is to determine in vitro the effect of different viscosupplements on the rheological properties of synovial fluid. Methods A detailed rheological characterization of synovial fluid from 26 patients undergoing total knee arthroplasty (TKA) was performed. The rheological properties of the osteoarthritic synovial fluid added with viscosupplements were compared with those of the healthy human synovial fluid in order to assess the restorative effect on naturally occurring synovial fluid within the joint. Furthermore, the stability of rheological properties of synovial fluid mixed with viscosupplements in vitro over 8 weeks was analyzed. Results The addition of a cross-linked viscosupplement to OA synovial fluid was found to be more efficient than linear ones in improving the overall rheological properties of synovial fluid. The findings indicate that the rheological properties of SF mixed with VSs changed over 8 weeks. Conclusions The findings from this study will lead to a better understanding of the role of viscosupplements in joint lubrication and OA.
    Biomedical Engineering Letters. 1(4).

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