Laceyella sediminis sp. nov., a thermophilic bacterium isolated from a hot spring

Biotechnology Research Center, Faculty of Life Science and Technology, Kunming University of Science and Technology (KUST), Kunming, PR China.
International Journal of Systematic and Evolutionary Microbiology (Impact Factor: 2.51). 02/2011; 62(Pt 1):38-42. DOI: 10.1099/ijs.0.028282-0
Source: PubMed


A thermophilic bacterium, designated strain RHA1(T), was isolated from a sediment sample collected from a hot spring in Tengchong county, Yunnan province, south-west China, and was characterized by using a polyphasic approach. Based on its phenotypic and phylogenetic characteristics, strain RHA1(T) was affiliated to the genus Laceyella. The strain formed white aerial and yellow-white substrate mycelia, bearing single endospores on short sporophores. The cell-wall peptidoglycan contained meso-diaminopimelic acid. Whole-cell hydrolysates contained ribose and glucose. The major fatty acids were iso-C(15:0) (62.39%) and anteiso-C(15:0) (17.55%)(.) The predominant menaquinone was MK-9. The G+C content of the genomic DNA of strain RHA1(T) was 47.9 mol%. Based on DNA-DNA hybridization data, chemotaxonomic characteristics and differential physiological properties, strain RHA1(T) is considered to represent a novel species of the genus Laceyella, for which the name Laceyella sediminis sp. nov. is proposed; the type strain is RHA1(T) (=DSM 45263(T)=CCTCC AA 208058(T)).

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Available from: Wen-Jun Li, Jun 13, 2015
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    • "The genus Laceyella was proposed by Yoon et al. 2005 after a detailed polyphasic study and reclassification on the genus Thermoactinomyces [1]. At present the genus Laceyella has four recognized species namely; Laceyella putida [1], L. sacchari, type species of the genus Laceyella [1], Laceyella sediminis [2] and Laceyella tengchongensis [3]. Strain GS 1-1 is a Gram-positive bacteria and thermophilic bacteria. "
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    ABSTRACT: We report the 3.3-Mb draft genome of Laceyella sacchari strain GS 1-1, isolated from hot spring water sample, Chumathang, Leh, India. Draft genome of strain GS 1-1 consists of 3, 324, 316 bp with a G + C content of 48.8% and 3429 predicted protein coding genes and 75 RNAs. Geobacillus thermodenitrificans strain NG80-2, Geobacillus kaustophilus strain HTA426 and Geobacillus sp. Strain G11MC16 are the closest neighbors of the strain GS 1-1.
    Genomics Data 12/2014; 2:18–19. DOI:10.1016/j.gdata.2013.10.007
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    • "During our investigation of thermophilic micro-organisms in this geothermal field, three members of the family Thermoactinomycetaceae have been described in previous studies (Chen et al., 2012; Yu et al., 2012; Zhang et al., 2010), and Planifilum yunnanense, described by Zhang et al. (2007), was also isolated from this area. Here, we characterize another new member of the family Thermoactinomycetaceae on the basis of phylogenetic and polyphasic biochemical studies. "
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    ABSTRACT: Strains YIM 77562T and YIM 77580, two novel Gram-staining positive, filamentous bacterial isolates were recovered from the Rehai Geothermal Field, Tengchong, Yunnan province, south-west China. Good growth was observed at 50-55 °C and pH 7.0. Aerial mycelium was absent on all the media tested. Substrate mycelia were well-developed, long, with moderately flexuous and formed abundant single warty ornamented endospores. Phylogenetic analysis of the 16S rRNA gene sequences of the two strains indicated that they belong to the family Thermoactinomycetaceae. Similarity levels between the 16S rRNA gene sequences of the two strains and those of the type strains of Thermoactinomycetaceae members were 88.33-93.24 %; the highest sequence similarity was with Hazenella coriacea DSM 45707T. In both strains, the predominant menaquinone was MK-7, the diagnostic diamino acid was meso-diaminopimelic acid, and the major cellular fatty acids were iso-C14:0, iso-C15:0, and iso-C16:0. The major polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, unidentified polar lipid and unidentified phospholipid. The G+C contents of the genomic DNAs of strains YIM 77562T and YIM 77580 were 45.5 and 44.2 mol%, respectively. DNA-DNA relatedness data suggest that the two isolates represent a single species. Based on phylogenetic analyses and physiological and biochemical characteristics, it is proposed that the two strains represent a novel species in a new genus, Geothermomicrobium terrae gen. nov., sp. nov. and the type strain is YIM 77562T (= CCTCC AA 2011022T = JCM 18057T).
    International Journal of Systematic and Evolutionary Microbiology 06/2014; 64(Pt 9). DOI:10.1099/ijs.0.059766-0 · 2.51 Impact Factor
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    • "(Lacey and Cross 1989; Yoon et al. 2005; Chen et al. 2011 "
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    ABSTRACT: A novel filamentous bacterium, designated YIM 77831(T), was isolated from a geothermal soil sample collected at Rehai National Park, Tengchong, Yunnan province, south-west China. Growth occurred from 28 to 65 °C (optimum 50 °C), pH 6.0-8.0 (optimum pH 7.0). The strain formed branched substrate mycelia, endospores were produced on the substrate mycelium and aerial mycelium was not produced on any of the growth media tested. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain YIM 77831(T) was affiliated with the family Thermoactinomycetaceae. The stain YIM 77831(T) contained meso-diaminopimelic acid in the cell wall. Whole-cell hydrolysates contained glucose, galactose, mannose, ribose and rhamnose. The polar lipids were phosphatidylmethylethanolamine, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminophospholipid and four unknown phospholipids. The only menaquinone was MK-7. Major fatty acids were iso-C(15:0), anteiso-C(15:0) and anteiso-C(17:0). The G+C content was 55.6 mol%. On the basis of the morphological and chemotaxonomic characteristics as well as genotypic data, strain YIM 77831(T) represents a novel genus and species, Lihuaxuella thermophila gen. nov., sp. nov., in the family Thermoactinomycetaceae. The type strain is YIM 77831(T) (CCTCC AA 2011024(T) = JCM 18059(T)).
    Antonie van Leeuwenhoek 07/2012; 102(4):711-8. DOI:10.1007/s10482-012-9771-6 · 1.81 Impact Factor
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