Laceyella sediminis sp. nov., a thermophilic bacterium isolated from a hot spring.
ABSTRACT A thermophilic bacterium, designated strain RHA1(T), was isolated from a sediment sample collected from a hot spring in Tengchong county, Yunnan province, south-west China, and was characterized by using a polyphasic approach. Based on its phenotypic and phylogenetic characteristics, strain RHA1(T) was affiliated to the genus Laceyella. The strain formed white aerial and yellow-white substrate mycelia, bearing single endospores on short sporophores. The cell-wall peptidoglycan contained meso-diaminopimelic acid. Whole-cell hydrolysates contained ribose and glucose. The major fatty acids were iso-C(15:0) (62.39%) and anteiso-C(15:0) (17.55%)(.) The predominant menaquinone was MK-9. The G+C content of the genomic DNA of strain RHA1(T) was 47.9 mol%. Based on DNA-DNA hybridization data, chemotaxonomic characteristics and differential physiological properties, strain RHA1(T) is considered to represent a novel species of the genus Laceyella, for which the name Laceyella sediminis sp. nov. is proposed; the type strain is RHA1(T) (=DSM 45263(T)=CCTCC AA 208058(T)).
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ABSTRACT: Strains YIM 77562T and YIM 77580, two novel Gram-staining positive, filamentous bacterial isolates were recovered from the Rehai Geothermal Field, Tengchong, Yunnan province, south-west China. Good growth was observed at 50-55 °C and pH 7.0. Aerial mycelium was absent on all the media tested. Substrate mycelia were well-developed, long, with moderately flexuous and formed abundant single warty ornamented endospores. Phylogenetic analysis of the 16S rRNA gene sequences of the two strains indicated that they belong to the family Thermoactinomycetaceae. Similarity levels between the 16S rRNA gene sequences of the two strains and those of the type strains of Thermoactinomycetaceae members were 88.33-93.24 %; the highest sequence similarity was with Hazenella coriacea DSM 45707T. In both strains, the predominant menaquinone was MK-7, the diagnostic diamino acid was meso-diaminopimelic acid, and the major cellular fatty acids were iso-C14:0, iso-C15:0, and iso-C16:0. The major polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, unidentified polar lipid and unidentified phospholipid. The G+C contents of the genomic DNAs of strains YIM 77562T and YIM 77580 were 45.5 and 44.2 mol%, respectively. DNA-DNA relatedness data suggest that the two isolates represent a single species. Based on phylogenetic analyses and physiological and biochemical characteristics, it is proposed that the two strains represent a novel species in a new genus, Geothermomicrobium terrae gen. nov., sp. nov. and the type strain is YIM 77562T (= CCTCC AA 2011022T = JCM 18057T).International Journal of Systematic and Evolutionary Microbiology 06/2014; 64. DOI:10.1099/ijs.0.059766-0 · 2.80 Impact Factor
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ABSTRACT: A Gram-staining-positive, endospore-forming rod was independently isolated from clinical specimens in New York State, USA, once in 2009 and twice in 2011. The three isolates have identical 16S rRNA gene sequences and based on 16S rRNA gene sequence, are most closely related to Laceyella sediminis and L. sacchari (94.6% similarity). The partial 23S rRNA gene sequence of the three strains is also 100% identical. Maximum-likelihood phylogenetic analysis suggests that the new isolates belong to the family Thermoactinomycetaceae. Additional biochemical and phenotypic characteristics of the strains support the family designation and suggest that the three isolates represent a single species. In each of the strains, the predominant menaquinone is MK-7, the diagnostic diamino acid is meso-diaminopimelic acid, and the major cellular fatty acids are iso-C15:0, anteiso-C15:0, and iso-C13:0. The polar lipids are phosphatidylglycerol, diphosphatidylglycerol, phospatidylethanolamine, four unknown phospholipids, four unknown aminophospholipids and an unknown lipid. It is proposed that the novel isolates represent a single species within a new genus. The name Hazenella coriacea gen. nov., sp. nov. (type strain 23436T=DSM 45707T=LMG 27204T) is proposed.International Journal of Systematic and Evolutionary Microbiology 05/2013; DOI:10.1099/ijs.0.050914-0 · 2.80 Impact Factor
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ABSTRACT: We report the 3.3-Mb draft genome of Laceyella sacchari strain GS 1-1, isolated from hot spring water sample, Chumathang, Leh, India. Draft genome of strain GS 1-1 consists of 3, 324, 316 bp with a G + C content of 48.8% and 3429 predicted protein coding genes and 75 RNAs. Geobacillus thermodenitrificans strain NG80-2, Geobacillus kaustophilus strain HTA426 and Geobacillus sp. Strain G11MC16 are the closest neighbors of the strain GS 1-1.12/2014; 2:18–19. DOI:10.1016/j.gdata.2013.10.007