Article

A landmark-free morphometric staging system for the mouse limb bud.

EMBL-CRG Systems Biology Research Unit, Centre for Genomic Regulation, UPF, Calle Dr. Aiguader 88, Barcelona 08003, Spain.
Development (impact factor: 6.6). 03/2011; 138(6):1227-34. DOI:10.1242/dev.057547 pp.1227-34
Source: PubMed

ABSTRACT We have created a 2D morphometric analysis of the developing mouse hindlimb bud. This analysis has provided two useful resources for the study of limb development. First, a temporally accurate numerical description of shape changes during normal mouse limb development. Second, a web-based morphometric staging system, which has the advantage of being easy to use, and with a reproducibility of about ±2 hours. It allows users to upload a dorsal-view photo of a limb bud, draw a spline curve and thereby stage the bud within a couple of minutes. We describe how the system is constructed, its robustness to user variation and illustrate one application: the accurate tracking of spatiotemporal dynamics of gene expression patterns.

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Keywords

2D morphometric analysis
 
bud
 
developing mouse hindlimb bud
 
dorsal-view photo
 
gene expression patterns
 
limb bud
 
limb development
 
normal mouse limb development
 
reproducibility
 
spatiotemporal dynamics
 
spline curve
 
temporally accurate numerical description
 
user variation
 
users
 
web-based morphometric
 

Bernd Boehm