Article

Monolithic columns based on a poly(styrene-divinylbenzene-methacrylic acid) copolymer for capillary liquid chromatography of small organic molecules.

Charles University in Prague, Faculty of Science, Department of Analytical Chemistry, Albertov 6, 12843 Prague 2, Czech Republic.
Journal of chromatography. A (impact factor: 4.19). 01/2011; 1218(11):1544-7. DOI:10.1016/j.chroma.2011.01.042 pp.1544-7
Source: PubMed

ABSTRACT A very simple and readily performed method is described for the preparation of poly(styrene-divinylbenzene-methacrylic acid) monolithic columns for capillary liquid chromatography. The effect of the methacrylic acid content on the morphological and chromatographic properties has been investigated. Methacrylic acid is shown to be essential for isocratic separations of small organic analytes by capillary liquid chromatography. Column efficiencies of about 28,000 theoretical plates/m have been obtained for all the test compounds. The batch-to-batch and run-to-run repeatability of the retention times is better than 1.5%.

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Keywords

capillary liquid chromatography
 
Column efficiencies
 
essential
 
isocratic separations
 
Methacrylic acid
 
methacrylic acid content
 
poly(styrene-divinylbenzene-methacrylic acid)
 
run-to-run repeatability
 
simple
 
small organic analytes
 
test compounds