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Complete genome sequence of Slackia heliotrinireducens type strain (RHS 1T)

Standards in Genomic Sciences (Impact Factor: 3.17). 12/2009; 1(3):234-41. DOI: 10.4056/sigs.37633
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ABSTRACT Slackia heliotrinireducens (Lanigan 1983) Wade et al. 1999 is of phylogenetic interest because of its location in a genomically yet uncharted section of the family Coriobacteriaceae, within the deep branching Actinobacteria. Strain RHS 1(T) was originally isolated from the ruminal flora of a sheep. It is a proteolytic anaerobic coccus, able to reductively cleave pyrrolizidine alkaloids. Here we describe the features of this organism, together with the complete genome sequence, and annotation. This is the first complete genome sequence of the genus Slackia, and the 3,165,038 bp long single replicon genome with its 2798 protein-coding and 60 RNA genes is part of the Genomic Encyclopedia of Bacteria and Archaea project.

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Available from: Patrik D'haeseleer, Aug 29, 2015
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    • "At present, the complete genomes from Atopobium parvulum [39], Cryptobacterium curtum [40], Eggerthella lenta [41], Olsenella uli [42], and Slackia heliotrinireducens [43] are available. S. anaerobia has a smaller genome than E. lenta and S. heliotrinireducens (2,384,013 bp vs 3, 632,260 bp and 3,165,038 bp, respectively) but larger than A. parvulum, C. curtum, and O. uli (2,384,013 bp vs 1,543,805 bp, 1,617,804 bp, and 2,051,896 bp, respectively). "
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    • "Sl. heliotrinireducens is a Gram-positive anaerobic bacterium which can reduce nitrate to ammonia if there are electron donors (H2 or formate) in the system. This organism has also been reported to produce acetic acid and lactic acid, and contains a hydrogenase [99,100]. Bf. longum is a Gram-positive bacterium found as a symbiont in the human normal intestinal flora [101]. "
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    • "If not corrected, this can be as much as 35% of the sequences (Gomez-Alvarez et al., 2009; Quince et al., 2009). Recently, the overestimation of taxa was illustrated when a single genome generated hundreds of different sequence types, leading to recommendations of strict quality filtering and careful application of sequence difference cut-offs for grouping sequences into operational taxa (Pukall et al., 2009; Purushe et al., 2010). We have compared a pyrosequencing approach with study rumen fungi with parallel clone libraries of the internal transcribed spacer (ITS-1) gene region and found that, in general, the same community pattern is found using both techniques (S. "
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