Complete genome sequence of Kangiella koreensis type strain (SW-125T)

Standards in Genomic Sciences (Impact Factor: 3.17). 12/2009; 1(3):226-33. DOI: 10.4056/sigs.36635
Source: PubMed


Kangiella koreensis (Yoon et al. 2004) is the type species of the genus and is of phylogenetic interest because of the very isolated location of the genus Kangiella in the gammaproteobacterial order Oceanospirillales. K. koreensis SW-125(T) is a Gram-negative, non-motile, non-spore-forming bacterium isolated from tidal flat sediments at Daepo Beach, Yellow Sea, Korea. Here we describe the features of this organism, together with the complete genome sequence, and annotation. This is the first completed genome sequence from the genus Kangiella and only the fourth genome from the order Oceanospirillales. This 2,852,073 bp long single replicon genome with its 2647 protein-coding and 48 RNA genes is part of the Genomic Encyclopedia of Bacteria and Archaea project.

Download full-text


Available from: Johannes Sikorski, Oct 07, 2015
20 Reads
  • Source
    • "In the responsive fraction, 5% of the bacterial sequences were most closely related to Kangiella, a genus of non-motile, gram-negative Gammaproteobacteria (Yoon et al., 2004; Han et al., 2009) found in marine sediments (Romanenko et al., 2010). Cultured members of this group have not previously been shown to degrade complex carbon (Yoon et al., 2004; Jean et al., 2012) suggesting possible new roles for Kangiella either directly using lignocellulose or indirectly using lignocellulose-derived degradation by-products in the HBW marsh. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Carbon cycling by microbes has been recognized as the main mechanism of organic matter decomposition and export in coastal wetlands, yet very little is known about the functional diversity of specific groups of decomposers (e.g., bacteria) in salt marsh benthic trophic structure. Indeed, salt marsh sediment bacteria remain largely in a black box in terms of their diversity and functional roles within salt marsh benthic food web pathways. We used DNA stable isotope probing (SIP) utilizing (13)C-labeled lignocellulose as a proxy to evaluate the fate of macrophyte-derived carbon in benthic salt marsh bacterial communities. Overall, 146 bacterial species were detected using SIP, of which only 12 lineages were shared between enriched and non-enriched communities. Abundant groups from the (13)C-labeled community included Desulfosarcina, Spirochaeta, and Kangiella. This study is the first to use heavy-labeled lignocellulose to identify bacteria responsible for macrophyte carbon utilization in salt marsh sediments and will allow future studies to target specific lineages to elucidate their role in salt marsh carbon cycling and ultimately aid our understanding of the potential of salt marshes to store carbon.
    Frontiers in Microbiology 06/2014; 5:263. DOI:10.3389/fmicb.2014.00263 · 3.99 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Alcohol dehydrogenases (ADHs) are a group of dehydrogenase enzymes that facilitate the interconversion between alcohols and aldehydes or ketones with the reduction of NAD(+) to NADH. In bacteria, some alcohol dehydrogenases catalyze the opposite reaction as part of fermentation to ensure a constant supply of NAD(+). The adh gene from Kangiella koreensis was cloned and the protein (KkADH) was expressed, purified and crystallized. A KkADH crystal diffracted to 2.5 Å resolution and belonged to the monoclinic space group P21, with unit-cell parameters a = 94.1, b = 80.9, c = 115.6 Å, β = 111.9°. Four monomers were present in the asymmetric unit, with a corresponding VM of 2.55 Å(3) Da(-1) and a solvent content of 51.8%.
    Acta Crystallographica Section F Structural Biology and Crystallization Communications 09/2013; 69(Pt 9):1037-40. DOI:10.1107/S1744309113022008 · 0.53 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: A recombinant alcohol dehydrogenase (ADH) from Kangiella koreensis was purified as a 40 kDa dimer with a specific activity of 21.3 nmol min−1 mg−1, a K m of 1.8 μM, and a k cat of 1.7 min−1 for all-trans-retinal using NADH as cofactor. The enzyme showed activity for all-trans-retinol using NAD+ as a cofactor. The reaction conditions for all-trans-retinol production were optimal at pH 6.5 and 60 °C, 2 g enzyme l−1, and 2,200 mg all-trans-retinal l−1 in the presence of 5 % (v/v) methanol, 1 % (w/v) hydroquinone, and 10 mM NADH. Under optimized conditions, the ADH produced 600 mg all-trans-retinol l−1 after 3 h, with a conversion yield of 27.3 % (w/w) and a productivity of 200 mg l−1 h−1. This is the first report of the characterization of a bacterial ADH for all-trans-retinal and the biotechnological production of all-trans-retinol using ADH.
    Biotechnology Letters 12/2014; 37(4). DOI:10.1007/s10529-014-1740-x · 1.59 Impact Factor