Indentation Versus Tensile Measurements of Young's Modulus for Soft Biological Tissues

Department of Surgical and Radiological Science, School of Veterinary Medicine, University of California Davis, Davis, California 95616, USA.
Tissue Engineering Part B Reviews (Impact Factor: 4.64). 02/2011; 17(3):155-64. DOI: 10.1089/ten.TEB.2010.0520
Source: PubMed

ABSTRACT In this review, we compare the reported values of Young's modulus (YM) obtained from indentation and tensile deformations of soft biological tissues. When the method of deformation is ignored, YM values for any given tissue typically span several orders of magnitude. If the method of deformation is considered, then a consistent and less ambiguous result emerges. On average, YM values for soft tissues are consistently lower when obtained by indentation deformations. We discuss the implications and potential impact of this finding.

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Available from: Christopher J Murphy, Sep 28, 2015
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    • "The atomic force microscope (AFM) as a powerful instrument has been widely used to measure the biomechanical properties of living cells. Due to the nanoscale precision of 3D imaging and real-time force measurement, AFM has been developed into a versatile tool allowing researchers to image and indent individual biological specimens in physiological solution (Kasas et al., 2013; Kuznetsova et al., 2007; McKee et al., 2011; Rettler et al., 2013). For instance, Girasole and co-workers employed the combination of atomic force microscope (AFM) and lateral force microscope (LFM) to study the metal-induced cytotoxic effects on cells. "
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    Micron 08/2015; 79. DOI:10.1016/j.micron.2015.08.005 · 1.99 Impact Factor
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    • "In addition, we have also shown that the explicit results can also access other hyperelastic properties, e.g., the locking stretch k m in the Arruda– Boyce model. It is of notice that Sneddon's solution has been widely used to interpret the indentation of biological soft tissues and cells as well [35] [36] [37] [38] [39] [40] [41] by assuming that the indented material is homogeneous and the contact radius is much smaller than the curvature radius of the indented surface. In these cases, the same issue regarding the applicability of the Sneddon's solution exists when the indented soft tissue or cell undergoes finite deformation. "
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    • "The cells also had lower attachment affinity to PDMS than to tissue culture polystyrene (TCPS), which was in agreement with previous studies by other groups [49] [50]. However , we chose PDMS as our culture substrate because its Young's modulus of $0.6–2 MPa [51] [52] [53] is closer to the reported range of Young's modulus of normal cornea and Descemet's membrane, which varies from 0.029 MPa [54] to 2.57 MPa [55] and from 0.29 MPa [56] to 3 MPa [57], respectively. The wide range of reported Young's modulus is due to the difference in the measurement modalities. "
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