Genetic Analysis of Hedgehog Signaling in Ventral Body Wall Development and the Onset of Omphalocele Formation

Global COE, Cell Fate Regulation Research and Education Unit, Department of Organ Formation, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto, Japan.
PLoS ONE (Impact Factor: 3.23). 01/2011; 6(1):e16260. DOI: 10.1371/journal.pone.0016260
Source: PubMed


An omphalocele is one of the major ventral body wall malformations and is characterized by abnormally herniated viscera from the body trunk. It has been frequently found to be associated with other structural malformations, such as genitourinary malformations and digit abnormalities. In spite of its clinical importance, the etiology of omphalocele formation is still controversial. Hedgehog (Hh) signaling is one of the essential growth factor signaling pathways involved in the formation of the limbs and urogenital system. However, the relationship between Hh signaling and ventral body wall formation remains unclear.
To gain insight into the roles of Hh signaling in ventral body wall formation and its malformation, we analyzed phenotypes of mouse mutants of Sonic hedgehog (Shh), GLI-Kruppel family member 3 (Gli3) and Aristaless-like homeobox 4 (Alx4). Introduction of additional Alx4(Lst) mutations into the Gli3(Xt/Xt) background resulted in various degrees of severe omphalocele and pubic diastasis. In addition, loss of a single Shh allele restored the omphalocele and pubic symphysis of Gli3(Xt/+); Alx4(Lst/Lst) embryos. We also observed ectopic Hh activity in the ventral body wall region of Gli3(Xt/Xt) embryos. Moreover, tamoxifen-inducible gain-of-function experiments to induce ectopic Hh signaling revealed Hh signal dose-dependent formation of omphaloceles.
We suggest that one of the possible causes of omphalocele and pubic diastasis is ectopically-induced Hh signaling. To our knowledge, this would be the first demonstration of the involvement of Hh signaling in ventral body wall malformation and the genetic rescue of omphalocele phenotypes.

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Available from: Ryuma Haraguchi, Oct 13, 2015
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    • "ymal progenitors for GT pro - trusion . The lateral mesoderm derived from the caudal part of the primitive streak contributes to the cloaca known as the pericloacal mesenchyme ( PCM ) [ Cambray and Wilson , 2007 ] . PCM cells are proposed to contribute to the lower body wall , bladder , internal urethra , and GT [ Haraguchi et al . , 2007 , 2012 ; Matsumaru et al . , 2011 ; Su - zuki et al . , 2012 ] . Origin of the GT mesenchyme was traced by analyzing the hedgehog - responsive cells . The results indicate that the hedgehog - responsive PCM cells adjacent to the umbilical cord contribute to the bladder and the dorsal GT mesenchyme . Also , loss of SHH signal - ing results in hypoplastic bladder , urethr"
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