Article

Five challenges to bringing single-molecule force spectroscopy into living cells.

Universite catholique de Louvain, Institute of Condensed Matter and Nanosciences, Louvain-la-Neuve, Belgium.
Nature Methods (Impact Factor: 23.57). 02/2011; 8(2):123-7. DOI: 10.1038/nmeth0211-123
Source: PubMed

ABSTRACT In recent years, single-molecule force spectroscopy techniques have been used to study how inter- and intramolecular interactions control the assembly and functional state of biomolecular machinery in vitro. Here we discuss the problems and challenges that need to be addressed to bring these technologies into living cells and to learn how cellular machinery is controlled in vivo.

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