Chemical Composition and antiproliferative activity of essential oil from the leaves of a medicinal herb, Levisticum officinale, against UMSCC1 head and neck squamous carcinoma cells.
ABSTRACT Oral squamous cell carcinoma (OSCC) is a challenging disease with a high mortality rate. Natural products represent a valuable source for the development of novel anticancer drugs. We investigated the cytotoxic potential of essential oil from the leaves of a medicinal plant, Levisticum officinale (lovage) on head and neck squamous carcinoma cells (HNSCC).
Cytotoxicity of lovage essential oil was investigated on the HNSCC cell line, UMSCC1. Additionally, we performed pharmacogenomics analyses.
Lovage essential oil extract had an IC₅₀ value of 292.6 μg/ml. Genes involved in apoptosis, cancer, cellular growth and cell cycle regulation were the most prominently affected in microarray analyses. The three pathways to be most significantly regulated were extracellular signal-regulated kinase 5 (ERK5) signaling, integrin-linked kinase (ILK) signaling, virus entry via endocytic pathways and p53 signaling.
Levisticum officinale essential oil inhibits human HNSCC cell growth.
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ABSTRACT: Goniothalamin (GTN), a plant bioactive styryl-lactone, is a natural product with potent anti-tumorigenesis effects for several types of cancer. Nonetheless, the anticancer effect of GTN has not been examined in oral cancer. The present study was designed to evaluate its potential anticancer effects in an oral squamous cell carcinoma (OSCC) model and to determine the possible mechanisms with respect to apoptosis, DNA damage, reactive oxygen species (ROS) induction, and mitochondrial membrane potential. Our data demonstrated that cell proliferation was significantly inhibited by GTN in Ca9-22 OSCC cancer cells in concentration- and time-dependent manners (p<0.05). For cell cycle and apoptotic effects of GTN-treated Ca9-22 cancer cells, the sub-G1 population and annexin V-intensity significantly increased in a concentration-dependent manner (p<0.001). For the analysis of DNA double strand breaks, γH2AX intensity significantly increased in GTN-treated Ca9-22 cancer cells in concentration-response relationship (p<0.05). Moreover, GTN significantly induced intracellular ROS levels in Ca9-22 cancer cells in a concentration- and time-dependent manner (p<0.05). For membrane depolarization of mitochondria, the DiOC(2)(3) (3,3'-diethyloxacarbocyanine iodide) intensity of GTN-treated Ca9-22 cancer cells was significantly decreased in concentration- and time-dependent relationships (p<0.001). Taken together, these results suggest that the anticancer effect of GTN against oral cancer cells is valid and GTN-induced growth inhibition and apoptosis influence the downstream cascade including ROS induction, DNA damage, and mitochondria membrane depolarization. Therefore, GTN has potential as a chemotherapeutic agent against oral cancer.Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 06/2012; 747(2):253-8. · 3.90 Impact Factor
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ABSTRACT: The immunostimulating properties of inactivated parapoxvirus ovis (iPPVO) have long been demonstrated in vivo and in vitro, yet the biological and molecular mechanisms involved remain largely unknown. We herein report that intraperitoneal inoculation of iPPVO in mice results in stimulation of several events of the innate immune response. Increased interferon I (IFN-I) activity was demonstrated in sera of mice treated with iPPVO at 6 and 12h post-inoculation (hpi), and enhanced expression of IFN-γ (15-fold increase) and IL-12 (6-fold) mRNA was detected in the spleen of treated mice at 24 and 48hpi, respectively. A significant increase in neutrophil activity (p<0.01) was observed at 6hpi in the blood of iPPVO treated mice. In addition, increased phagocytic activity by peritoneal macrophages of iPPVO-treated mice (p<0.01) was detected in vivo (from 24 to 72hpi) and in vitro (12 to 96hpi). Bactericidal activity of sera mice treated with iPPVO against Escherichia coli was also increased (p<0.05) at 24 and 72hpi. Taken together, these results demonstrate that iPPVO administration leads to a transient stimulation of selected innate immune mechanisms, likely contributing to the immunostimulant effects observed against viral and bacterial infections in vivo.Cellular Immunology 03/2014; 289(1-2):36-41. · 1.74 Impact Factor