Article

Investigation of the effects of the chronic administration of some antihypertensive drugs on enzymatic and non-enzymatic oxidant/antioxidant parameters in rat ovarian tissue.

Ministry of Health, Obstetrics and Gynecology Hospital, Iğdır, Turkey.
Gynecological Endocrinology (Impact Factor: 1.14). 11/2011; 27(11):895-9. DOI: 10.3109/09513590.2010.551564
Source: PubMed

ABSTRACT In this study, effects of chronic antihypertensive drug (clonidine, methyldopa, amlodipine, ramipril, and rilmenidine) treatment on antioxidant-oxidant parameters were investigated in rat ovarian tissue.
Chronic drug administration for 30 days and at the end, biochemical examinations (total glutathione (tGSH), glutathione peroxidase (GPO), glutathione reductase (GR), glutathione s-transferase (GST), superoxide dismutase (SOD), nitric oxide (NO), catalase (CAT), malondialdehyde (MDA), and myeloperoxidase (MPO) analyses) were performed.
The levels of glutathione (GSH) and NO, and the activities of GPO, GR, GST, SOD, and CAT were measured the lowest in ramiprile group. Also in ramiprile group, the level of MDA and the activity of MPO was the highest.
We divided the drugs into four groups according to their biochemical side effect potentials in ovarian tissue: (I) Drugs which have no clear negative effect on ovarian tissue: clonidine, rilmenidine; (II) Drugs which have mild negative effect on ovarian tissue: methyldopa; (III) Drugs which have moderate negative effect on ovarian tissue: amlodipine; (IV) Drugs which have severe negative effect on ovarian tissue: ramipril. These data might be useful in the selection of the least toxic antihypertensive drug in pregnant and/or normal females.

0 Followers
 · 
106 Views
  • [Show abstract] [Hide abstract]
    ABSTRACT: The oxidant/antioxidant balance in healthy tissues is maintained with a predominance of antioxidants. Various factors that can lead to tissue damage disrupt the oxidant/antioxidant balance in favor of oxidants. In this study, disruptions of the oxidant/antioxidant balance in favor of oxidants were found to be a consequence of the over-consumption of antioxidants. For this reason, antioxidants are considered to be of importance in the prevention and treatment of various types of tissue damage that are aggravated by stress.
    02/2013; 45(1):47-9. DOI:10.5152/eajm.2013.08
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Aims: Hypertension is known to cause potentially fatal kidney damage. However, it is unclear whether long-term use of antihypertensive drugs establishes any toxic effect on the kidney. This study investigate whether clonidine, methyldopa, rilmenidine, amlodipine and ramipril establish nephrotoxic effects in rats. Materials and methods: Methyldopa, clonidine, rilmenidine, amlodipine and ramipril were administered orally to rat groups for three months. At the end of that period, rats were sacrificed by decapitation, the kidneys extracted and biochemical and histopathological analyses performed. Results: The experimental results revealed that methyldopa and ramipril slightly increased Malondialdehyde (MDA) and Myeloperoxidase (MPO) levels in rat kidney tissue and slightly reduced total glutathione (GSH). Rilmenidine increased MDA and MPO levels more than methyldopa and ramipril, but less than clonidine and amlodipine. Severe glomerular cellularity, hyalinization, tubulointerstitial inflammation and tubular necrosis were encountered in the clonidine and amlodipine groups, in which MDA and MPO were highest and GSH lowest. These histopathological findings were moderate in the rilmenidine group and mild in the methyldopa and ramipril groups. In addition to these histopathological findings, mild interstitial fibrosis and increased mesenchymal matrix were observed in the amlodipine group and increased mesenchymal matrix alone in the clonidine group. Conclusions: Ramipril and methyldopa were identified as drugs causing mild nephrotoxicity, rilmenidine moderate nephrotoxicity and amlodipine and clonidine severe nephrotoxicity. Key words: Antihypertensive agents, kidney, toxicity, rat.
    Acta Medica Mediterranea 01/2014; 30: 515(30: 515):30: 515. DOI:10.13140/2.1.1994.3047 · 1.05 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Background In this study, we investigated the effects of treatment with chronic antihypertensive drugs (clonidine, methyldopa, amlodipine, ramipril and rilmenidine) on oxidant-antioxidant parameters and toxic effects on DNA in rat uterus tissue. In addition, uterus tissues were examined histopathologically. Materials and Methods A total of 36 albino Wistar rats were divided into the following six groups: 0.075 mg/kg clonidine group; 100 mg/kg methyldopa group; 2 mg/kg amlodipine group; 2.5 mg/kg ramipril group; 0.5 mg/kg rilmenidine group; and the healthy group. Rats underwent chronic drug administration for 30 days and at the end, biochemical and histopathological examinations were performed. All data were subjected to one-way ANOVA test. Results We divided these drugs into the following three groups according to their effects on rat uteri: (I) mild negative effects (clonidine), (II) moderate negative effects (rilmenidine, methyldopa) and (III) drugs which had severe negative effects (amlodipine, ramipril). ConclusionThese data may help with selection of antihypertensive drugs, in order to determine which drugs have the lowest toxicity in pregnant and non-pregnant (pre-pregnancy) women.
    International journal of fertility & sterility 07/2011; 5(2):96-103. · 0.47 Impact Factor