[Show abstract][Hide abstract] ABSTRACT: Genes encoding RNA-binding proteins are diverse and abundant in eukaryotic genomes. Although some have been shown to have roles in post-transcriptional regulation of the expression of specific genes, few of these proteins have been studied systematically. We have used an affinity tag to isolate each of the five members of the Puf family of RNA-binding proteins in Saccharomyces cerevisiae and DNA microarrays to comprehensively identify the associated mRNAs. Distinct groups of 40-220 different mRNAs with striking common themes in the functions and subcellular localization of the proteins they encode are associated with each of the five Puf proteins: Puf3p binds nearly exclusively to cytoplasmic mRNAs that encode mitochondrial proteins; Puf1p and Puf2p interact preferentially with mRNAs encoding membrane-associated proteins; Puf4p preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors; and Puf5p is associated with mRNAs encoding chromatin modifiers and components of the spindle pole body. We identified distinct sequence motifs in the 3'-untranslated regions of the mRNAs bound by Puf3p, Puf4p, and Puf5p. Three-hybrid assays confirmed the role of these motifs in specific RNA-protein interactions in vivo. The results suggest that combinatorial tagging of transcripts by specific RNA-binding proteins may be a general mechanism for coordinated control of the localization, translation, and decay of mRNAs and thus an integral part of the global gene expression program.
[Show abstract][Hide abstract] ABSTRACT: C. 2005: Towards DNA-aided biogeography: An example from Tetramorium ants (Hyme-noptera, Formicidae). — Ann. Zool. Fennici 42: 23–35. The increasing level of fine-scale systematics in insects requires sophisticated mor-phometric tools for species identification. As a consequence, regional species lists and biogeographic data tend to be ambiguous. We explore the use of DNA techniques in an example of palearctic ants of the myrmicine genus Tetramorium, in which morphol-ogy-based determination is difficult and frequently controversial. Several chorological facts are uncovered by the combined use of morphological characters and mitochon-drial DNA sequences: Tetramorium moravicum is reported from Bulgaria and Ukraine, T. hungaricum from Austria and Bulgaria, while sequence comparisons question published records of T. semilaeve and T. forte in Eastern Europe. These findings have permitted us to delineate a biogeographic framework for T. forte, T. moravicum, T. semilaeve and T. hungaricum.
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