Spatial light interference microscopy (SLIM)

Department of Electrical and Computer Engineering, Beckman Institute for Advanced Science & Technology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
Optics Express (Impact Factor: 3.49). 01/2011; 19(2):1016-26. DOI: 10.1364/OE.19.001016
Source: PubMed


We present spatial light interference microscopy (SLIM) as a new optical microscopy technique, capable of measuring nanoscale structures and dynamics in live cells via interferometry. SLIM combines two classic ideas in light imaging: Zernike's phase contrast microscopy, which renders high contrast intensity images of transparent specimens, and Gabor's holography, where the phase information from the object is recorded. Thus, SLIM reveals the intrinsic contrast of cell structures and, in addition, renders quantitative optical path-length maps across the sample. The resulting topographic accuracy is comparable to that of atomic force microscopy, while the acquisition speed is 1,000 times higher. We illustrate the novel insight into cell dynamics via SLIM by experiments on primary cell cultures from the rat brain. SLIM is implemented as an add-on module to an existing phase contrast microscope, which may prove instrumental in impacting the light microscopy field at a large scale.

Download full-text


Available from: Gabriel Popescu, Sep 30, 2015
1 Follower
36 Reads
  • Source
    • "The SLM can be used in both illuminating and imaging paths of the optical microscope, where it ensures a structured specimen illumination, or amplitude and phase modulation of the spatial spectrum, respectively [4]. Using these phase modulation techniques, the spatial light interference microscopy (SLIM) [5] [6] and the spiral phase contrast imaging [7] were presented as powerful techniques capable of measuring nanoscale structures and dynamics in live cells or enhancing standard phase contrast methods. A variability of operations provided by the SLM was also used to design the universal microscope, that operatively combines the standard techniques of optical microscopy [8] [9]. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Incoherent correlation microscopy is recently discovered technique for digital imaging of three-dimensional objects in a quasi-monochromatic spatially incoherent light. Its operation is based on wavefront division carried out by a spatial light modulator and capturing correlation recordings of the observed scene. To achieve image reconstruction, at least a partial overlapping of the signal and reference waves created by the spatial light modulator is necessary. In the known experimental configurations, the overlapping of interfering beams is strongly reduced in off-axis areas of the object and the image can be reconstructed only in a very small portion of the field of view provided by the used microscope objective lens. Here, we propose and successfully demonstrate modified experimental system working with two-component relay optics inserted between the microscope objective and the spatial light modulator and providing full overlapping of correlated beams in all areas of the field of view of the objective lens. The benefits and applicability of the proposed system design are clearly demonstrated on the imaging of the USAF resolution targets.
    Journal of the European Optical Society Rapid Publications 02/2013; 8. DOI:10.2971/jeos.2013.13011] · 1.23 Impact Factor
  • Source
    • "Compared to other traditional optical techniques such as phase contrast microscopy or differential interference contrast microscopy, quantitative phase microscopy (QPM) has been developed to visualize and quantitatively analyze the distribution of phase shift of transmitted light through a specimen with nanometer resolution [1–3]. Since the amount of phase shift indicates the optical path difference (which contains the information of both the thickness and refractive index of the specimen), the QPM technique has been used to discern diverse cellular information under biophysical conditions such as the structural fluctuation of erythrocyte [4,5], cell growth depending on the cell cycle [6] and the measurement of refractive indices of intracellular materials [7,8]. In recent years, numerous novel techniques using QPM have been developed to enable a stable and quantitative measurement for long-term cellular dynamics using low-coherent illumination [7,8] and diffraction phase microscopy [9]. "
    [Show abstract] [Hide abstract]
    ABSTRACT: There is a need for a noninvasive technique to monitor living pluripotent stem cell condition without any labeling. We present an optical imaging technique that is able to capture information about optical path difference through the cell and cell adhesion properties simultaneously using a combination of quantitative phase microscopy (QPM) and interference reflection microscopy (IRM) techniques. As a novel application of QPM and IRM, this multimodal imaging technique demonstrated its ability to distinguish the undifferentiated status of human induced pluripotent stem (hiPS) cells quantitatively based on the variation of optical path difference between the nucleus and cytoplasm as well as hiPS cell-specific cell adhesion properties.
    Biomedical Optics Express 09/2012; 3(9):2175-83. DOI:10.1364/BOE.3.002175 · 3.65 Impact Factor
  • Source
    • "To obtain the quantitative phase profile of the sample, various IPM setups were previously suggested [1–3,15–17]. These include wide-field digital interferometry configuration that is based on an off-axis holographic setup [1], and enables us to obtain the quantitative phase profile of the sample by using only a single interferogram. "
    [Show abstract] [Hide abstract]
    ABSTRACT: We present analysis tools which are formulated using wide-field interferometric phase microscopy measurements, and show their ability to uniquely quantify the life cycle of live cancer cells. These parameters are based directly on the optical path delay profile of the sample and do not necessitate decoupling the refractive index and the thickness in the cell interferometric phase profile, and thus can be calculated using a single-frame acquisition. To demonstrate the use of these parameters, we have constructed a wide-field interferometric phase microscopy setup and closely traced the full lifecycle of HeLa cancer cells. These initial results show the potential of the parameters to distinguish between the different phases of the cell lifecycle, as well others biological phenomena.
    Biomedical Optics Express 08/2012; 3(8):1757-73. DOI:10.1364/BOE.3.001757 · 3.65 Impact Factor
Show more