Article

Micropilot: automation of fluorescence microscopy-based imaging for systems biology

Advanced Light Microscopy Facility, European Molecular Biology Laboratory, Heidelberg, Germany.
Nature Methods (Impact Factor: 23.57). 01/2011; 8(3):246-9. DOI: 10.1038/nmeth.1558
Source: PubMed

ABSTRACT Quantitative microscopy relies on imaging of large cell numbers but is often hampered by time-consuming manual selection of specific cells. The 'Micropilot' software automatically detects cells of interest and launches complex imaging experiments including three-dimensional multicolor time-lapse or fluorescence recovery after photobleaching in live cells. In three independent experimental setups this allowed us to statistically analyze biological processes in detail and is thus a powerful tool for systems biology.

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