Article

Label-free single-molecule detection of DNA-hybridization kinetics with a carbon nanotube field-effect transistor.

Department of Electrical Engineering, Columbia University, New York, New York 10027, USA.
Nature Nanotechnology (Impact Factor: 31.17). 02/2011; 6(2):126-32. DOI: 10.1038/nnano.2010.275
Source: PubMed

ABSTRACT Single-molecule measurements of biomolecules can provide information about the molecular interactions and kinetics that are hidden in ensemble measurements. However, there is a requirement for techniques with improved sensitivity and time resolution for use in exploring biomolecular systems with fast dynamics. Here, we report the detection of DNA hybridization at the single-molecule level using a carbon nanotube field-effect transistor. By covalently attaching a single-stranded probe DNA sequence to a point defect in a carbon nanotube, we are able to measure two-level fluctuations in the conductance of the nanotube in the presence of a complementary DNA target. The kinetics of the system are studied as a function of temperature, allowing the measurement of rate constants, melting curves and activation energies for different sequences and target concentrations. The kinetics demonstrate non-Arrhenius behaviour, in agreement with DNA hybridization experiments using fluorescence correlation spectroscopy. This technique is label-free and could be used to probe single-molecule dynamics at microsecond timescales.

1 Bookmark
 · 
331 Views
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: This article focuses on recent advances and developments of field effect transistor (FET) devices for detecting DNA recognition events such as hybridization, SNP genotyping and primer extension. The unique features of FET biosensors highlight the potential advantages for high-throughput detection of DNA molecules in a label-free manner. In particular, FET devices represent a potential platform for the development of the next-generation DNA sequence instruments based on semiconductor technology. We also review an emerging class of FET devices that use nanomaterials such as silicon nanowires and single-walled carbon nanotubes as a gate channel for the ultrasensitive detection of biological analytes.
    Current Physical Chemistry. 01/2011; 1(4):276-291.
  • [Show abstract] [Hide abstract]
    ABSTRACT: A single-walled carbon nanotube (SWCNT) in a field-effect transistor (FET) configuration provides an ideal electronic path for label-free detection of nucleic acid hybridization. The simultaneous influence of more than one response mechanism in hybridization detection causes a variation in electrical parameters such as conductance, transconductance, threshold voltage and hysteresis gap. The channel length (L) dependence of each of these parameters necessitates the need to include them when interpreting the effect of L on the response to hybridization. Using the definitions of intrinsic effective mobility (µe) and device field-effect mobility (µf), two new parameters were defined to interpret the effect of L on the FET response to hybridization. Our results indicate that FETs with ≈300 µm long SWCNT exhibited the most appreciable response to hybridization, which complied with the variation trend in response to the newly defined parameters.
    Beilstein Journal of Nanotechnology 01/2014; 5:2081-91. · 2.33 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Instrumental limitations such as bulkiness and high cost prevent the fluorescence technique from becoming ubiquitous for point-of-care deoxyribonucleic acid (DNA) detection and other in-field molecular diagnostics applications. The complimentary metal-oxide-semiconductor (CMOS) technology, as benefited from process scaling, provides several advanced capabilities such as high integration density, high-resolution signal processing, and low power consumption, enabling sensitive, integrated, and low-cost fluorescence analytical platforms. In this paper, CMOS time-resolved, contact, and multispectral imaging are reviewed. Recently reported CMOS fluorescence analysis microsystem prototypes are surveyed to highlight the present state of the art.
    Sensors 11/2014; 14(11):20602-20619. · 2.05 Impact Factor

Full-text (2 Sources)

Download
109 Downloads
Available from
May 22, 2014