Label-free single-molecule detection of DNA-hybridization kinetics with a carbon nanotube field-effect transistor

Department of Electrical Engineering, Columbia University, New York, New York 10027, USA.
Nature Nanotechnology (Impact Factor: 34.05). 02/2011; 6(2):126-32. DOI: 10.1038/nnano.2010.275
Source: PubMed

ABSTRACT Single-molecule measurements of biomolecules can provide information about the molecular interactions and kinetics that are hidden in ensemble measurements. However, there is a requirement for techniques with improved sensitivity and time resolution for use in exploring biomolecular systems with fast dynamics. Here, we report the detection of DNA hybridization at the single-molecule level using a carbon nanotube field-effect transistor. By covalently attaching a single-stranded probe DNA sequence to a point defect in a carbon nanotube, we are able to measure two-level fluctuations in the conductance of the nanotube in the presence of a complementary DNA target. The kinetics of the system are studied as a function of temperature, allowing the measurement of rate constants, melting curves and activation energies for different sequences and target concentrations. The kinetics demonstrate non-Arrhenius behaviour, in agreement with DNA hybridization experiments using fluorescence correlation spectroscopy. This technique is label-free and could be used to probe single-molecule dynamics at microsecond timescales.

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Available from: Chien-Yang Chiu, Sep 28, 2015
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    • "DNA biosensors are sensing devices that consist of oligonucleotide of DNA as biological recognition elements and incorporated with physiochemical transducer for converting DNA hybridization events into a useful analytical signal [9]. Several types of transducers for detecting DNA hybridization events have been reported such as the electrochemical transducer [10] [11] optical transducer [12] [13] piezoelectric transducer [14] and electrical transducer [15]. Electrochemical sensor has been gaining attraction in the diagnostic detection because of not only offering many benefits, including low cost of production, simplicity of protocol, portable, sensitivity and selectivity, and easily integrated and miniaturization but also can convert the hybridization event into a direct electrical signal [16] [17]. "
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    ABSTRACT: In this study, a disposable screen-printed gold electrode (SPGE) utilized of silicon nanowires (SiNWs) and gold nanoparticles as sensing material was fabricated for detection of DNA oligomers related to dengue virus. Firstly, SiNWs/AuNPs-SPGE was developed by the dispersion of SiNWs in 3-Aminopropyltriethoxysilane (APTES, 0.5 %) onto bare SPGE. Secondly, the AuNPs decoration on SiNWs-SPGE surface was functionalized using dithiopropionic acid (DTPA) through a self-assembly monolayer (SAM) technique. The electrochemical response of methylene blue (MB) as a redox indicator towards synthetic DNA oligomer after hybridization on SiNWs/AuNPs-SPGE was recorded by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques. The results demonstrated that the reduction peak current of MB was significantly decreased after DNA hybridization process. In addition, the developed biosensor showed a good storage stability and could achieve a linear range of 1.0 × 10-11-1.0 × 10-7 M (R= 0.98) with the detection limit of 1.63× 10-12 M.
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    • "This process can contribute to the hyperchromic effect [4]. Lower thermal stability was observed for decamers hybridized on the individual carbon nanotube [15] and for DNA linked to gold nanoparticles [46]. Most likely, the decrease of the thermal stability of the double-stranded polymer hybridized on the solid surfaces or nanoparticles is a general observation, which occurs due to interactions between the polymers and the surface. "
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    ABSTRACT: Hybridization of homopolynucleotide poly(rC) adsorbed to the carbon nanotube surface with poly(rI) free in solution has been studied by absorption spectroscopy and molecular dynamics method. It was found that hybridization on the nanotube surface has a slow kinetics, the behavior of which differs essentially from fast hybridization of free polymers. The duplex obtained is characterized with the reduced thermostability and a lower hyperchromic coefficient than it was observed when the duplex was formed in the absence of the nanotube. These features point to the imperfectness in the structure of the duplex hybridized on the nanotube surface. Computer simulation showed that the strong interaction of nitrogen bases with the nanotube surface weakens significantly hybridization of two complementary oligomers, as the surface prevents the necessary conformational mobility of the polymer to be hybridized.
    Nanoscale Research Letters 04/2014; 9(1):157. DOI:10.1186/1556-276X-9-157 · 2.78 Impact Factor
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    • "In the last decade, the convergence of nanotechnology with biology and medicine and the ability to fabricate structures using standard wafer-scale semiconductor processing techniques has shown an emerging and evolving interest in the development of new classes of rapid, sensitive, and reliable biosensors devices, such as nanowires and carbon nanotubes [1][2][3][4][5]. These devices could have a great impact on many application fields related to our life: from health-care and environment, to food production and bio-warfare thanks to their properties of electrical, label-free and real time readout. "
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