Toxoplasma gondii and Neospora caninum in wildlife: Common parasites in Belgian foxes and Cervidae?

Scientific Institute of Public Health, Communicable and Infectious Diseases, National Reference Center for Toxoplasmosis, Engelandstraat 642, B1180 Brussels, Belgium.
Veterinary Parasitology (Impact Factor: 2.46). 12/2010; 178(1-2):64-9. DOI: 10.1016/j.vetpar.2010.12.016
Source: PubMed


Sera from Cervidae were tested for the presence of antibodies against Neospora caninum using ELISA; and against Toxoplasma gondii using SAG1-ELISA and a commercially available agglutination test. The T. gondii seroprevalence was 52% (38/73) in roe deer (Capreolus capreolus), 0% in bred fallow deer (0/4) (Dama dama) and red deer (0/7) (Cervus elaphus). We found 2.7% of the roe deer samples and none of the bred deer samples positive for N. caninum. Brain samples from wild roe deer, red deer and red foxes (Vulpes vulpes) were tested for the presence of T. gondii and N. caninum DNA using multiplex real-time PCR. We detected T. gondii in 18.8% (57/304) of the red foxes and in 1 of the 33 deer samples. N. caninum was found in 6.6% of the red foxes and in 2 roe deer samples. Twenty-six of the T. gondii positive DNA extracts from the red fox samples were genotyped. Twenty-five were type II and only one was found to be type III.

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    • "In summary, our results show no distinctive genotype dispersion pattern for the study area, but indicate some differences in the prevalence of types among wild animal species. The genotype distribution of T. gondii in southwestern Spain may be more diverse than that indicated by studies in other European countries (De Craeye et al. 2011). It is possible that the small sample size tested in some cases could also diminish the likelihood of detecting landscape patterns. "
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    ABSTRACT: Abstract We used PCR to detect Toxoplasma gondii in the principal game species in southwestern Spain. We detected T. gondii in 32.2% of animals tested. Prevalences varied from 14.7% in wild boar (Sus scrofa) to 51.2% in red fox (Vulpes vulpes). The most prevalent genotype was type II (50.0%), followed by type III (20.6%) and type I (5.9%). Mixed infections (11.8%) were detected in wild boar (types I+III) and red fox (types II+III). Polymorphic strains (11.8%) were detected in several species. The high prevalence and the genetic variability shown could have implications for infection of farm animals and humans.
    Journal of wildlife diseases 01/2015; 51(1):233-238. DOI:10.7589/2013-09-233. · 1.36 Impact Factor
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    • "BEL 26 0 25 1 0 Fox RFLP (3) + MS (6) [De Craeye et al., 2011] "
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    ABSTRACT: Toxoplasma gondii is an extremely sucessfull protozoal parasite which infects almost all mamalian species including humans. Approximately 30% of the human population worldwide is chronically infected with T. gondii. In general, human infection is asymptomatic but the parasite may induce severe disease in fetuses and immunocompromised patients. In addition, T. gondii may cause sight-threatening posterior uveitis in immunocompetent patients. Apart from few exceptions, humans acquire T. gondii from animals. Both, the oral uptake of T. gondii oocysts released by specific hosts, i.e. felidae, and of cysts persisting in muscle cells of animals result in human toxoplasmosis. In the present review, we discuss recent new data on the cell biology of T. gondii and parasite diversity in animals. In addition, we focus on the impact of these various parasite strains and their different virulence on the clinical outcome of human congenital toxoplasmosis and T. gondii uveitis.
    International Journal of Medical Microbiology 09/2014; 304(7). DOI:10.1016/j.ijmm.2014.09.002 · 3.61 Impact Factor
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    • "). than the prevalence of T. gondii DNA (6%). From Belgian red fox brain samples, De Craeye et al. (2011) also found a higher prevalence of T. gondii (18.8%) than of N. caninum (6.6%), but they used a moresensitive real-time PCR. In contrast, in the Czech Republic a molecular survey showed a higher prevalence of N. caninum (4.6%) than of T. gondii (1.3%; Hurková and Modry´2006 "
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    ABSTRACT: Abstract Brain samples from 182 red foxes (Vulpes vulpes) from Romania were examined using a standard PCR technique. Results provide evidence of Toxoplasma gondii (11 foxes = 6.0%) and Neospora caninum (1 fox = 0.5%) DNA in red foxes from Romania. No coinfections were found.
    Journal of wildlife diseases 05/2014; 50(3). DOI:10.7589/2013-07-167 · 1.36 Impact Factor
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