Sudden Onset of Atrial Flutter During Continuous Venovenous Hemodialysis

Department of Anesthesia and Perioperative Care, University of California, San Francisco, Veterans Affairs Medical Center, San Francisco, CA 94121, USA.
Journal of cardiothoracic and vascular anesthesia (Impact Factor: 1.46). 01/2011; 26(3):535-6. DOI: 10.1053/j.jvca.2010.11.008
Source: PubMed
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    ABSTRACT: Summary Leaf disc transformation-regeneration technique was applied to the drought tolerant wild relative of cultivated tomato,Lycopersicon chilense, using a plasmid construct which contained the coding sequences of neomycin phosphotransferase (NPTII) and chloramphenicol acetyltransferase (CAT) genes. The two genotypes used, LA2747 and LA1930, showed a distinct difference in their aptitude to transformation; a higher success rate was obtained for the first genotype in every stage of the process. Shoots were formed on the regeneration medium containing 100 µg/ml kanamycin through direct or indirect organogenesis. Root formation became only possible when the concentration of kanamycin was reduced to 50 µg/ml. Expression of chloramphenicol acetyltransferase gene was observed in all of the kanamycin-screened plants after they matured; the activity of the gene was absent or low in some of the young plants. The presence of the CAT gene in transgenic plants was further confirmed by Southern blot analysis. Although transgenic plants grew to maturity, they did not produce fruit, owing to the self incompatibility ofL. chilense.
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    ABSTRACT: Conditions for plant regeneration from explants of tomato (Lycopersicon esculentum) cv. UC82B were studied for optimizing transformation procedure. The best regeneration rate was obtained from cotyledon explants from 8-10-d-old seedlings on a modified Murashige and Skoog medium (1962) with 0·5 mg dm-3 zeatin and 0·5 mg dm-3 indolylacetic acid. Tomato cultivars (UC82B, Castone, Fl Ferline, Monalbo) and a Lycopersicon peruvkmum 'CMV sel. INRA' were studied. The cultivarUC82B and the wild Lycopersicon species showed an efficient shoot regeneration potential.Early events in the transformation of tomato cotyledons were analysed using an Agrobacterium tumefaciens strain carrying a binary vector with an nptII (pnos) gene and a reporter GUS-intron (p35S) chimeric gene. Two days after infection, GUS activity appeared specifically at the cut surface. Subepidermal cells were more susceptible to transformation than epidermal cells. When selection for kanamycin resistance was applied 2 d after inoculation, transformed cells were efficiently recovered. Preculture with feeder cells stimulated cell transformation, but reducedregeneration capacity from transformed cells. The optimal transformation rate was observed witha time of preculture of 1 and 2 d. Transformation events for two tomato cultivars (UC82B and Monalbo) occurred at the same rate as 55% of the inoculated explants developed kanamycin resistant calli. However, transformed plants were obtained at different rates of 8% and 14% for cv. Monalbo and cv. UC82B.
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    ABSTRACT: An improved protocol forAgrobacterium-mediated transformation of the tomato cultivar Moneymaker was developed by examining the effects of six different factors on the efficiency of transformation. Explant size, explant orientation, gelling agent and plate sealant were found to affect transformation efficiency. Two other factors, type of explant (hypocotyl or cotyledon) and frequency of transfer to fresh selective regeneration medium, did not have any effect on transformation efficiency. By combining the best treatments for each factor, an average transformation efficiency of 10.6% was obtained for Moneymaker.
    Plant Cell Reports 12/1996; 16(3-4):235-40. DOI:10.1007/BF01890875 · 3.07 Impact Factor
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