Green synthesis of gold nanoparticles using Nyctanthes arbortristis flower extract.

Biomaterials and Tissue Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, 781039, Assam, India.
Bioprocess and Biosystems Engineering (Impact Factor: 1.82). 06/2011; 34(5):615-9. DOI: 10.1007/s00449-010-0510-y
Source: PubMed

ABSTRACT The present study explores the reducing and capping potentials of ethanolic flower extract of the plant Nyctanthes arbortristis for the synthesis of gold nanoparticles. The extract at different volume fractions were stirred with HAuCl4 aqueous solution at 80 °C for 30 min. The UV-Vis spectroscopic analysis of the reaction products confirmed successful reduction of Au(3+) ions to gold nanoparticles. Transmission electron microscope (TEM) revealed dominant spherical morphology of the gold nanoparticles with an average diameter of 19.8 ± 5.0 nm. X-ray diffraction (XRD) study confirmed crystalline nature of the synthesized particles. Fourier transform infra-red (FTIR) and nuclear magnetic resonance (NMR) analysis of the purified and lyophilized gold nanoparticles confirmed the surface adsorption of biomolecules during preparation and caused long-term (6 months) stability. Low reaction temperature (25 °C) favored anisotropy. The strong reducing power of the flower extract can also be tested in the green synthesis of other metallic nanoparticles.

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    ABSTRACT: Here we report the synthesis of silver nanoparticles using ethanolic flower extract of Nyctanthes arbortristis, UVvisible spectra and TEM indicated the successful formation of silver nanoparticles. Crystalline nature of the silver nanoparticles was confirmed by X-ray diffraction. Fourier Transform Infra-Red Spectroscopy analysis established the capping of the synthesized silver nanoparticles with phytochemicals naturally occurring in the ethanolic flower extract of N. arbortristis. The synthesized silver nanoparticles showed antibacterial activity against the pathogenic strain of Escherichia coli MTCC 443. Furthermore, cytotoxicity of the silver nanoparticles was tested on mouse fibroblastic cell line (L929) and found to be non-toxic, which thus proved their biocompatibility. Antibacterial activity and cytotoxicity assay carried out in this study open up an important perspective of the synthesized silver nanoparticles. Copyright © 2014 Elsevier B.V. All rights reserved.
    Materials Science and Engineering C 04/2015; 46:463-9. DOI:10.1016/j.msec.2014.10.069 · 2.74 Impact Factor
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    ABSTRACT: This review focuses on the green synthesis of silver nanoparticles using various plant sources. Nano biotechnology focus on the use of living organisms plants for engineering nanoparticles and its biomedical, pharmaceutical applications. Plants extracts provide rapid, cost effective and eco-friendly sources for fabrication of metallic nanoparticles. Green biological method of synthesizing nanoparticles has materialized as alternative to overcome the curb of conventional methods such as synthesized by several physical and chemical methods including chemical reduction of ions in aqueous solution with or without stabilizing agent and reduction in inverse micelles or thermal decomposition in organic solvents. Employing plants towards synthesis of nanoparticles has advantageous over non biological methods as with the presence of broad variability of bio-molecules in plants can act as capping and reducing agents and thus increases the rate of reduction and stabilization of nanoparticles. Thus biosynthesized metallic nanoparticles of variable size and shape have broad potential applications in life and science. Keyword: Biosynthesized Nanoparticles, Green Source, Biofabrication, Ecofriendly, Applications


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May 15, 2014