Comparison of two Mycoplasma genitalium real-time PCR detection methodologies.

Department of Microbiology & Infectious Diseases, The Royal Women's Hospital, Melbourne, Australia.
Journal of clinical microbiology (Impact Factor: 4.23). 03/2011; 49(3):1140-2. DOI: 10.1128/JCM.02328-10
Source: PubMed

ABSTRACT Established in-house quantitative PCR (qPCR) assays to detect the Mycoplasma genitalium adhesion protein (MgPa) and the 16S rRNA gene were found to be comparable for screening purposes, with a kappa value of 0.97 (95% confidence interval [CI], 0.94 to 1.01) and no difference in bacterial load quantified (P = 0.4399).

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