Comparison of Two Mycoplasma genitalium Real-Time PCR Detection Methodologies

Department of Microbiology & Infectious Diseases, The Royal Women's Hospital, Melbourne, Australia.
Journal of clinical microbiology (Impact Factor: 4.23). 03/2011; 49(3):1140-2. DOI: 10.1128/JCM.02328-10
Source: PubMed

ABSTRACT Established in-house quantitative PCR (qPCR) assays to detect the Mycoplasma genitalium adhesion protein (MgPa) and the 16S rRNA gene were found to be comparable for screening purposes, with a kappa value of 0.97 (95% confidence interval [CI], 0.94 to 1.01) and no difference in bacterial load quantified (P = 0.4399).

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    ABSTRACT: Mycoplasma genitalium (M. genitalium) was first isolated from the urethral swabs of two symptomatic men with urethritis in 1980. Published prevalence rates vary greatly between populations studied. A number of urogenital conditions have been ascribed to M. genitalium, which is recognised to cause a sexually transmitted infection. The association of M. genitalium with non-specific urethritis is now well established, but the evidence supporting its role in both male and female infertility remains inconclusive. Laboratory methods are challenging and there is a lack of test standardisation. The recommended treatment of the infection is azithromycin as a single 1 gm dose. However, in recent years a macrolide resistance has been observed. More studies are required to establish the clinical importance of M. genitalium in urogenital conditions, particularly infertility, and to establish the role for screening and treatment in high-risk populations.
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    Asian Pacific journal of cancer prevention: APJCP 06/2013; 14(6):3425-8. DOI:10.7314/APJCP.2013.14.6.3425 · 1.50 Impact Factor

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