Article

Terriglobus saanensis sp. nov., an acidobacterium isolated from tundra soil.

Finnish Forest Research Institute, Eteläranta 55, FI-96300 Rovaniemi, Finland.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY (impact factor: 2.11). 12/2010; 61(Pt 8):1823-8. DOI:10.1099/ijs.0.026005-0 pp.1823-8
Source: PubMed

ABSTRACT Two aerobic bacterial strains, designated SP1PR4(T) and SP1PR5, were isolated from tundra soil samples collected from Saana fjeld, North-western Finland (69° 03' N 20° 50' E). Cells of both strains were Gram-negative, non-motile rods. Phylogenetic analysis indicated that the strains belong to the genus Terriglobus in subdivision 1 of the phylum Acidobacteria. Strains SP1PR4(T) and SP1PR5 shared identical BOX and ERIC fingerprints and 99.7 % 16S rRNA gene similarity indicating that, together with their identical physiological features, these strains are members of the same species. The 16S rRNA gene sequence similarity of SP1PR4(T) and SP1PR5 with Terriglobus roseus DSM 18391(T) was 97.1 %. A low DNA-DNA hybridization value (<20 %) and rpoB gene sequence similarity (83.6 %) with T. roseus DSM 18391(T) indicated that the tundra soil isolates represent novel members of the genus Terriglobus. Strains SP1PR4(T) and SP1PR5 grew at pH 4.5-7.5 and 4-30 °C. Sugars were the preferred growth substrates. The major cellular fatty acids were iso-C(15 : 0), C(16 : 1)ω7c, iso-C(13 : 0) and C(16 : 0). The DNA G+C content of strain SP1PR4(T) was 57.3 mol%. Based on phylogenetic, chemotaxonomic and physiological analyses, the name Terriglobus saanensis sp. nov. is proposed to accommodate the two strains; the type strain is SP1PR4(T) ( = DSM 23119(T)  = ATCC BAA-1853(T)).

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    Article: A robust PCR primer design platform applied to the detection of Acidobacteria Group 1 in soil.
    Jason D Gans, John Dunbar, Stephanie A Eichorst, La Verne Gallegos-Graves, Murray Wolinsky, Cheryl R Kuske
    [show abstract] [hide abstract]
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    Nucleic Acids Research 03/2012; 40(12):e96. · 8.03 Impact Factor
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Keywords

16S rRNA gene sequence similarity
 
chemotaxonomic
 
DNA G+C content
 
ERIC fingerprints
 
genus Terriglobus
 
identical physiological features
 
low DNA-DNA hybridization value
 
major cellular fatty acids
 
name Terriglobus saanensis sp
 
phylogenetic
 
Phylogenetic analysis
 
physiological analyses
 
preferred growth substrates
 
rpoB gene sequence similarity
 
subdivision 1
 
Terriglobus roseus DSM 18391(T)
 
tundra soil
 
tundra soil samples