Triennial Growth Symposium: Important roles for L-glutamine in swine nutrition and production

Department of Animal Science and of Veterinary Integrative Biosciences, Texas A&M University, College Station, 77843, USA.
Journal of Animal Science (Impact Factor: 2.11). 12/2010; 89(7):2017-30. DOI: 10.2527/jas.2010-3614
Source: PubMed


L-Glutamine (Gln) has traditionally not been considered a nutrient needed in diets for livestock species or even mentioned in classic animal nutrition textbooks. This is due to previous technical difficulties in Gln analysis and the unsubstantiated assumption that animals can synthesize sufficient amounts of Gln to meet their needs. Consequently, the current (1998) version of NRC does not recommend dietary Gln requirements for swine. This lack of knowledge about Gln nutrition has contributed to suboptimal efficiency of global pig production. Because of recent advances in research, Gln is now known to be an abundant AA in physiological fluids and proteins and a key regulator of gene expression. Additionally, Gln can regulate cell signaling via the mammalian target of rapamycin pathway, adenosine monophosphate-activated protein kinase, extracellular signal-related kinase, Jun kinase, mitogen-activated protein kinase, and nitric oxide. The exquisite integration of Gln-dependent regulatory networks has profound effects on cell proliferation, differentiation, migration, metabolism, homeostasis, survival, and function. As a result of translating basic research into practice, dietary supplementation with 1% Gln maintains gut health and prevents intestinal dysfunction in low-birth-weight or early-weaned piglets while increasing their growth performance and survival. In addition, supplementing 1% Gln to a corn- and soybean-meal-based diet between d 90 and 114 of gestation ameliorates fetal growth retardation in gilts and reduces preweaning mortality of piglets. Furthermore, dietary supplementation with 1% Gln enhances milk production by lactating sows. Thus, adequate amounts of dietary Gln, a major nutrient, are necessary to support the maximum growth, development, and production performance of swine.

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Available from: Junjun Wang, Nov 15, 2015
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    • "Besides its role in protein synthesis, Gln is the most versatile AA in cellular metabolism, intervening in several Gln-dependent regulatory pathways indispensable for cell proliferation, differentiation, migration and metabolism, besides being involved in purine and pyrimidine synthesis and gene expression regulation. Gln is also a major energy source for rapidly proliferating cells, including enterocytes, lymphocytes and intestinal mucosal cells (Wu et al., 2011), up-regulate antioxidant genes expression, prevents oxidant-induced apoptosis (Brasse-Lagnel et al., 2009), and modulates intestinal oxidative status, being precursor of glutathione, an important antioxidant molecule (Cheng et al., 2011). "
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    ABSTRACT: A study was undertaken to evaluate dietary glutamine supplementation effects on gilthead sea bream performance, intestinal nutrient absorption capacity, hepatic and intestinal glutamine metabolism and oxidative status. For that purpose gilthead sea bream juveniles (mean weight 13.0g) were fed four isolipidic (18% lipid) and isonitrogenous (43% protein) diets supplemented with 0, 0.5, 1 and 2% glutamine for 6weeks. Fish performance, body composition and intestinal nutrient absorption capacity were not affected by dietary glutamine levels. Hepatic and intestinal glutaminase (GlNase), glutamine synthetase (GSase), alanine aminotransferase, aspartate aminotransferase and glutamate dehydrogenase activities were also unaffected by dietary glutamine supplementation. In the intestine GlNase activity was higher and GSase/GlNase ratio was two-fold lower than in the liver, suggesting a higher use of glutamine for energy production by the intestine than by the liver. The liver showed higher catalase and glucose-6-phosphate dehydrogenase activities, while the intestine presented higher glutathione peroxidase and glutathione reductase activities and oxidised glutathione content, which seems to reveal a higher glutathione dependency of the intestinal antioxidant response. Total and reduced glutathione contents in liver and intestine and superoxide dismutase activity in the intestine were enhanced by dietary glutamine, though lipid peroxidation values were not affected. Overall, differences between liver and intestine glutamine metabolism and antioxidant response were identified and the potential of dietary glutamine supplementation to gilthead sea bream's antioxidant response was elucidated.
    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 09/2015; 191. DOI:10.1016/j.cbpa.2015.09.012 · 1.97 Impact Factor
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    • "If hepatic release is reduced, as observed Fig. 2 Rates of amino acid accretion in fetal pigs. Data are compiled from Wu et al. (1999) during dexamethasone-induced parturition, glutamine plays a central role in fetal carbon and nitrogen metabolism and exhibits the highest fetal/maternal plasma ratio among all amino acids in pigs (Wu et al. 2011). Throughout most of the gestation, there is an interorgan exchange of glutamine and glutamate between the placenta and fetal liver. "
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    ABSTRACT: The present review focuses on the physiological functions of glutamate-glutamine exchange involving placental amino acid transport and umbilical amino acid uptake in mammals (particularly in sows), with special emphasis on the associated regulating mechanisms. Glutamate plus glutamine are among the most abundant and the most utilized amino acids in fetus during late gestation. During pregnancy, amino acids, notably as precursors of macromolecules including proteins and nucleotides are involved in fetal development and growth. Amino acid concentrations in fetus are generally higher than in the mother. Among amino acids, the transport and metabolism of glutamate and glutamine during fetal development exhibit characteristics that clearly emphasize the importance of the interaction between the placenta and the fetal liver. Glutamate is quite remarkable among amino acids, which originate from the placenta, and is cleared from fetal plasma. In addition, the flux of glutamate through the placenta from the fetal plasma is highly correlated with the umbilical glutamate delivery rate. Glutamine plays a central role in fetal carbon and nitrogen metabolism and exhibits one of the highest fetal/maternal plasma ratio among all amino acids in human and other mammals. Glutamate is taken up by placenta from the fetal circulation and then converted to glutamine before being released back into the fetal circulation. Works are required on the glutamate-glutamine metabolism during late pregnancy in physiological and pathophysiological situations since such works may help to improve fetal growth and development both in humans and other mammals. Indeed, glutamine supplementation appears to ameliorate fetal growth retardation in sows and reduces preweaning mortality of piglets.
    Amino Acids 11/2014; 47(1). DOI:10.1007/s00726-014-1861-5 · 3.29 Impact Factor
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    • "Glutamate and Gln are major metabolic fuels for the mammalian small intestine (Rezaei et al. 2013a; Wu 1998) and represent the most abundant free and protein-bound AA in milk of all mammals (Davis et al. 1994a, b; Wu and Knabe 1994; DeSantiago et al. 1999; Ramirez et al. 2001), and these AA are considered to be essential during the first days of life of the suckling newborn (Rezaei et al. 2013b; Wu et al. 2011b). In sows and rats, mammary uptake of Glu and Gln is quantitatively the highest of all AA (Viña and Williamson 1981; Trottier et al. 1997). "
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    ABSTRACT: Amino acids (AA) are not only building blocks of protein but are also key regulators of metabolic pathways in animals. Understanding the fate of AA is crucial to optimize utilization of AA for milk protein synthesis and, therefore, to reduce inefficiencies of nutrient utilization during lactation. By understanding the functional role of AA metabolism in mammary tissue, we can uncover pathways and molecular targets to improve AA utilization by mothers and neonates during the lactation period. The major objective of this article is to highlight recent advances in mammary AA transport, metabolism and utilization. Such knowledge will aid in refining dietary requirements of AA for lactating mammals, including women, sows and cows.
    Amino Acids 09/2014; 46(11). DOI:10.1007/s00726-014-1818-8 · 3.29 Impact Factor
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