[The role of p38 on the differentiation of MSCs to myoblasts].

State Key Laboratory of Tranuma, Burns and Combined Injury, Institute of Combined Injury of PLA, College of Preventive Medicine, Third Military Medical University, Chongqing 400038.
Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology 11/2004; 20(4):345-8.
Source: PubMed


Inducing mesenchymal stem cells (MSCs) differentiate to myoblasts with 5-azacytidine(5-Aza-CR), investigating the expression of Myf5 and the role of the signal transduction case of p38 in all the course of differentiation.
Seperating and purifying bone marrow-derived MSCs, inducing MSCs differentiation to myoblasts with 10 micromol/L 5-Aza-CR, assaying the gene expression time of Myf5 with RT-PCR method, the antigen expression of myosion with immunohistochemistray method and observing the changes of the activity of phosphorylation p38 before and after inhibited by SB203580 with Western-blot method.
MSCs begin to express Myf5 delaied to the 9th day after inhibited by SB203580. Some of MSCs express myosion at the 7th day after induced; The phosphorylation p38 activity of MSCs enhanced after induced by 5-Aza-CR but obviously decreased after inhibited by SB203580.
MSCs can express myogenic regulator factors and orientation differentiate to myoblasts after induced by 5-Aza-CR, p38 really have a positive signal transduction effection in this course.

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