A novel sensitive electrochemical DNA biosensor for assaying of anticancer drug leuprolide and its adsorptive stripping voltammetric determination
Ankara University, Faculty of Pharmacy, Department of Analytical Chemistry, 06100 Tandogan, Ankara, Turkey. Talanta
(Impact Factor: 3.55).
01/2011; 83(3):780-8. DOI: 10.1016/j.talanta.2010.10.049
The anticancer drug, leuprolide (LPR) bound to double-stranded fish sperm DNA (dsDNA) which was immobilized onto the surface of an anodically activated pencil graphite electrode (PGE), was employed for designing a sensitive biosensor. The interaction of leuprolide (LPR) with double-stranded DNA (dsDNA) immobilized onto pencil graphite electrode (PGE) have been studied by electrochemical methods. The mechanism of the interaction was investigated and confirmed by differential pulse voltammetry using two different interaction methods; at the PGE surface and in the solution phase. The decrease in the guanine oxidation peak current was used as an indicator for the interaction in acetate buffer at pH 4.80. The response was optimized with respect to accumulation time, potential, drug concentration, and reproducibility for both interaction methods. The linear response was obtained in the range of 0.20-6.00 ppm LPR concentration with a detection limit of 0.06 ppm on DNA modified PGE and between 0.20 and 1.00 ppm concentration range with detection limit of 0.04 ppm for interaction in solution phase method. LPR showed an irreversible oxidation behavior at all investigated pH values on a bare PGE. Differential pulse adsorptive stripping (AdSDPV) voltammetric method was developed for the determination of LPR. Under these conditions, the current showed a linear dependence with concentration within a range of 0.005-0.20 ppm with a detection limit of 0.0014 ppm. Each determination method was fully validated and applied for the analysis of LPR in its pharmaceutical dosage form.
Available from: Sevinc Kurbanoglu
- "Electrochemical DNA biosensors enable the study of the interaction of DNA immobilized on the electrode surface with analytes in solution. Binding of drugs to DNA and a general DNA damage has been described in literature through the variation of the electrochemical signal of guanine or adenine        . To illustrate, in a recent study the interaction was occurred between DNA and mitomycin-C on electrode surface, and it was observed that the guanine signal was higher with bare electrode than DNA-modified one . "
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ABSTRACT: Interaction of an antipsychotic agent, aripiprazole, with calf thymus double stranded deoxyribonucleic acid, ct-dsDNA, was investigated by differential pulse voltammetry (using two methods; interaction on the dsDNA modified electrode surface and in solution phase) and UV–VIS spectrophotometry. The binding constant between dsDNA and aripiprazole, K, about 3 × 105 M−1 was obtained spectrophotometrically in 0.1 M acetate buffer, at pH 4.7. Moreover, the DNA-drug association interaction was confirmed by the differential pulse (DP) voltammetric and spectrophotometric investigations of the systems aripiprazole – polyGuanine (polyG) and aripiprazole – polyAdenine (polyA) in solution phase. The interaction between aripiprazole and damaged ct-dsDNA was also investigated using differential pulse voltammetry.
Electrochimica Acta 07/2015; 169. DOI:10.1016/j.electacta.2015.04.087 · 4.50 Impact Factor
Available from: Shou-Qing Liu
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ABSTRACT: The human metabolic process is a series of reactions between biomolecules in nature. Vitamin B 6 helps protein metabolism and supports DNA repair against free radical damage. Here we reported on studying the electron transfer between DNA and vitamin B 6 by observing the electro-catalytic behaviors of a ssDNA-modified electrode towards vitamin B 6 and determine the quantitative content of vitamin B 6 using the ssDNA-modified electrode. The electron transfer coefficient β was evaluated to be 0.36(±0.02), the standard rate constant k 0 was estimated to be 3.4(±0.1)×10 -4 cm s -1 and the electron transfer number for the rate-determining step is equal to 1. The diffusion coefficient for vitamin B 6 was found to be 4.2(±0.1)×10 -5 cm 2 s -1 . The peak currents depend linearly on the concentration of vitamin B 6 from 0.10 to 6.00 mM with R= 0.99952 (n=8) and SD=0.07008. The linear regression equation was C (mM) = 0.03245 × Ip (µA) -0.04049 with the detection limit of 0.040(±0.001) mM. The determination for the real tablets yielded a good mean recovery of 99.46% with a relative standard deviation of 5.36%. Thus, the method is expected to find its applications in monitoring the content of vitamin B 6 in medical industry.
International journal of electrochemical science 01/2012; 7(1). · 1.50 Impact Factor
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ABSTRACT: A simple and inexpensive methodology was used to develop a novel electrochemical sensor for the determination of Sudan II. The interaction of Sudan II with salmon sperm ds-DNA on the surface of salmon sperm ds-DNA-modified pencil graphite electrode (PGE) and in solution phase was studied, using differential pulse voltammetry. The difference between adenine and guanine signals of the ds-DNA after and before interaction with Sudan II was directly proportional to Sudan II concentration, which used for quantitative inspections. Using PGE, a linear calibration curve (R(2)=0.9958) was observed with 0.5-6.0 μg mL(-1) Sudan II. Furthermore, the LOD of 0.4 μg mL(-1) and linear range between 0.5 and 4.0 μg mL(-1) were achieved in solution phase. In the second part, Sudan II was determined on a pretreated pencil graphite electrode by means of adsorptive stripping differential pulse voltammetry. The peak current was linearly dependent on Sudan II concentration over the range of 0.0015-0.30 μg mL(-1), with a detection limit of 0.00007 μg mL(-1) Sudan II. Both ds-DNA-modified PGE and PPGE were applied to analyze Sudan II in real samples.
Talanta 01/2012; 88:244-51. DOI:10.1016/j.talanta.2011.10.038 · 3.55 Impact Factor
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