Decidual CD4+CD25+CD127dim/- regulatory T cells in patients with unexplained recurrent spontaneous miscarriage.
ABSTRACT To investigate the frequency and function of CD4(+)CD25(+)CD127(dim/-) regulatory T (Treg) cells in decidua of patients with unexplained recurrent spontaneous miscarriage (URSM).
The decidual lymphocytes from patients who experienced URSM and normal pregnant women (controls) were collected by Ficoll density gradient centrifugation. CD4(+)CD25(+)CD127(dim/-) Treg cells were isolated by magnetic cell sorting. The proportion of Treg cells and IL-10, TGF-β in Treg cells were determined by flow cytometry. Inhibitory effects of Treg cells on effecter T cells were detected with or without the presentation of anti-IL-10 antibodies and anti-TGF-β antibodies.
The frequency of CD4(+)CD25(+)CD127(dim/-) Treg cells was decreased in URSM decidua compared to controls (2.09%±0.86% vs. 2.97%±1.19%, p=0.005), and the expression of IL-10 and TGF-β in Treg cells was lower in the URSM group than in the control group (p=0.04 and p=0.01, respectively). Furthermore, the suppressive effect of CD4(+)CD25(+)CD127(dim/-) Treg cells on the proliferation of effector T cells was decreased in URSM decidua (p<0.05). Suppression was mediated predominantly through IL-10 and TGF-β in decidua.
Decreased frequency and immunosuppressive capacity of CD4(+)CD25(+)CD127(dim/-) Treg cells was found in URSM decidua. Treg cells inhibit proliferation of effector T cells mainly via IL-10 and TGF-β in URSM decidua.
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ABSTRACT: B lymphocytes are pleiotropic cells belonging to the adaptive arm of the immune system. Although B cells were classically regarded for their capacity to produce antibodies, in the recent years, several other functions were attributed to these cells. B cells can uptake, process and present antigens as well as produce several cytokines that further influence immunity.Mammalian pregnancies represent a fascinating phenomenon in which the maternal immune system must be able to 'tolerate' the semi-allogenic fetus while simultaneously protecting the mother and the fetus against external pathogens. This requires a finely regulated balance between immune activation and tolerance. In this regard, B cells and the antibodies they produced were shown to actively participate in both, pregnancy well-being as well as pregnancy-associated pathologies.We discuss here the currently available information concerning the role of B cells in the context of pregnancy.American Journal Of Reproductive Immunology 01/2013; · 3.32 Impact Factor
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