Isolation, Characterization, and Use for Plant Growth Promotion under Salt Stress, of ACC Deaminase-Producing Halotolerant Bacteria Derived from Coastal Soil

Department of Agricultural Chemistry, Chungbuk National University, Cheongju, Chungbuk, 361-763, Korea.
Journal of Microbiology and Biotechnology (Impact Factor: 1.53). 11/2010; 20(11):1577-84.
Source: PubMed


In total, 140 halotolerant bacterial strains were isolated from both the soil of barren fields and the rhizosphere of six naturally growing halophytic plants in the vicinity of the Yellow Sea, near the city of Incheon in the Republic of Korea. All of these strains were characterized for multiple plant growth promoting traits, such as the production of indole acetic acid (IAA), nitrogen fixation, phosphorus (P) and zinc (Zn) solubilization, thiosulfate (S2O3) oxidation, the production of ammonia (NH3), and the production of extracellular hydrolytic enzymes such as protease, chitinase, pectinase, cellulase, and lipase under in vitro conditions. From the original 140 strains tested, on the basis of the latter tests for plant growth promotional activity, 36 were selected for further examination. These 36 halotolerant bacterial strains were then tested for 1- aminocyclopropane-1-carboxylic acid (ACC) deaminase activity. Twenty-five of these were found to be positive, and to be exhibiting significantly varying levels of activity. 16S rRNA gene sequencing analyses of the 36 halotolerant strains showed that they belong to 10 different bacterial genera: Bacillus, Brevibacterium, Planococcus, Zhihengliuella, Halomonas, Exiguobacterium, Oceanimonas, Corynebacterium, Arthrobacter, and Micrococcus. Inoculation of the 14 halotolerant bacterial strains to ameliorate salt stress (150 mM NaCl) in canola plants produced an increase in root length of between 5.2% and 47.8%, and dry weight of between 16.2% and 43%, in comparison with the uninoculated positive controls. In particular, three of the bacteria, Brevibacterium epidermidis RS15, Micrococcus yunnanensis RS222, and Bacillus aryabhattai RS341, all showed more than 40% increase in root elongation and dry weight when compared with uninoculated saltstressed canola seedlings. These results indicate that certain halotolerant bacteria, isolated from coastal soils, have a real potential to enhance plant growth under saline stress, through the reduction of ethylene production via ACC deaminase activity.

Download full-text


Available from: Puneet Chauhan,

Click to see the full-text of:

Article: Isolation, Characterization, and Use for Plant Growth Promotion under Salt Stress, of ACC Deaminase-Producing Halotolerant Bacteria Derived from Coastal Soil

978.3 KB

See full-text
  • Source
    • "A few studies performed to date indicated that the species dominant in saline soils belonged to the genus Bacillus sp. Moreover, some typically halotolerant species were observed (e.g., Halobacillus, Halomonas and Brevibacterium halotolerans ) (Siddikee et al., 2010; Sgroy et al., 2009; Ramadoss et al., 2013). Reports on rhizosphere and endophytic microorganisms that demonstrate selective colonization of the roots of halophytes (which could indicate their preference for this particular group of plants) are lacking. "
    [Show abstract] [Hide abstract]
    ABSTRACT: The submitted work assumes that the abundance and diversity of endophytic and rhizosphere microorganisms co-existing with the halophytic plant Aster tripolium L. growing in a salty meadow in the vicinity of a soda factory (central Poland) represent unique populations of cultivable bacterial strains. Endophytic and rhizosphere bacteria were (i) isolated and identified based on 16S rDNA sequences; (ii) screened for nifH and acdS genes; and (iii) analyzed based on selected metabolic properties. Moreover, total microbial biomass and community structures of the roots (endophytes), rhizosphere and soil were evaluated using a cultivation-independent technique (PLFA) to characterize plant–microbial interactions under natural salt conditions. The identification of the isolated strains showed domination by Gram-positive bacteria (mostly Bacillus spp.) both in the rhizosphere (90.9%) and roots (72.7%) of A. tripolium. Rhizosphere bacterial strains exhibited broader metabolic capacities, while endophytes exhibited higher specificities for metabolic activity. The PLFA analysis showed that the total bacterial biomass decreased in the following order (rhizosphere < soil < endophytes) and confirmed the dominance of Gram-positive endophytic bacteria in the roots of the halophyte. The described strain collection provides a valuable basis for a subsequent applications of bacteria in improvement of site adaptation of plants in saline soils.
    Microbiological Research 01/2016; 182:68-79. DOI:10.1016/j.micres.2015.09.007 · 2.56 Impact Factor
  • Source
    • "The bacterium B.acillus aryabhattai, was first isolated from stratosphere air sample (Shivaji et al., 2009) and subsequently found in diverse ecological niches such as barren land, rhizosphere of halophytic plants, deep sea water of South China sea; characterized by various important traits such as plant growth promotion, tolerance to salt, heavy metal and UV radiation (Siddikee et al., 2011; Lee et al., 2012; Wen et al., 2015). In the present study, we report the degradation of OPs by B. aryabhattai strain SanPS1. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Organophosphate (OP) insecticides are widely used for controlling insect pests for better crop production in India. But indiscriminate use and lack of proper technical knowhow have resulted in contamination and pollution of large varieties of ecological niches. A novel bacterial strain designated as SanPS1, capable of utilizing chlorpyrifos and parathion, was isolated by enrichment culture from a soil sample of an agricultural field located at Narigram in Burdwan district of West Bengal, India. This novel Gram positive, endospore forming strain was identified as Bacillus aryabhattai based on 16S rDNA sequencing. The strain tolerated up to 500 μg mL−1 of chlorpyrifos and parathion and for both compounds optimal degradation was achieved at a concentration of 200 μg mL−1. The metabolites were identified by high performance liquid chromatography and gas chromatography-mass spectrometry analyses. We propose a degradation pathway of parathion for this strain through formation of 4-nitrophenol and 4-nitrocatechol intermediates. The strain could degrade approximately 56% of parathion in liquid mineral medium within 24 h at 37 °C.
    International Biodeterioration & Biodegradation 09/2015; 103. DOI:10.1016/j.ibiod.2015.05.006 · 2.13 Impact Factor
  • Source
    • "Application of maize increased also the relative abundance of phylotypes belonging to the genus Arthrobacter. Arthrobacter was described by Semenov et al. (2012) as a primary sequestrator with known cellulolytic activity (Siddikee et al., 2010). Proteobacteria whose population can fluctuate 'opportunistically', are well known to respond readily to labile C sources (Fierer et al., 2007). "
    [Show abstract] [Hide abstract]
    ABSTRACT: Agricultural practices affect the bacterial community structure in soil. It was hypothesized that agricultural practices would also affect the bacteria involved in the degradation of crop residue. Soil was sampled from four different agricultural practices, i.e. conventional agriculture on the flat or on beds, or conservation agriculture on the flat or on beds. Cultivating crops on the flat is done traditionally, but cultivating crops on beds was introduced so as to avoid water logging during the rainy season and its potential negative effect on yields. Soil from these four treatments was amended in the laboratory with maize residue (Zea mays L.) or its neutral detergent fibre (NDF) fraction, mostly consisting of (hemi) cellulose, and incubated aerobically for 14 days. Maize residue was applied to soil as it is left in the field in conservation agriculture and NDF was added to study which bacteria were favoured by application of (hemi) cellulose. Soil was incubated aerobically while the carbon mineralization and the bacterial population were monitored. On the one hand, the relative abundance of phylotypes belonging to bacterial groups that preferred low nutrient environments was higher in soil with conservation agriculture (e.g. Acidobacteria 17.6%, Planctomycetes 1.7% and Verrucomicrobia 1.5%) compared to conventional practices (Acidobacteria 11.8%, Planctomycetes 0.9% and Verrucomicrobia 0.4%). On the other hand, the relative abundance of phylotypes belonging to bacterial groups that preferred nutrient rich environments, such as Actinobacteria, showed an opposite trend. It was 11.9% in conservation agriculture and 16.2% in conventional practices. The relative abundance of Arthrobacter (Actinobacteria) and Bacillales more than doubled when maize residue was applied to soil compared to the unamended soil and that of Actinomycetales when maize or NDF was applied. Application of organic material reduced the relative abundance of a wide range of bacterial groups, e.g. Acidobacteria, Bacteroidetes, Planctomycetes and Verrucomicrobia. It was found that application of organic material favoured the same bacterial groups that were more abundant in the soil cultivated conventionally while it reduced those that were favoured in conservation agriculture
    Applied Soil Ecology 06/2015; 90. DOI:10.1016/j.apsoil.2015.01.010 · 2.64 Impact Factor
Show more