Article

A first comparative map of copy number variations in the sheep genome.

DIPROVAL, Sezione di Allevamenti Zootecnici, University of Bologna, Via F.lli Rosselli 107, 42123 Reggio Emilia, Italy.
Genomics (impact factor: 3.02). 03/2011; 97(3):158-65. DOI:10.1016/j.ygeno.2010.11.005 pp.158-65
Source: PubMed

ABSTRACT We carried out a cross species cattle-sheep array comparative genome hybridization experiment to identify copy number variations (CNVs) in the sheep genome analysing ewes of Italian dairy or dual-purpose breeds (Bagnolese, Comisana, Laticauda, Massese, Sarda, and Valle del Belice) using a tiling oligonucleotide array with ~385,000 probes designed on the bovine genome. We identified 135 CNV regions (CNVRs; 24 reported in more than one animal) covering ~10.5 Mb of the virtual sheep genome referred to the bovine genome (0.398%) with a mean and a median equal to 77.6 and 55.9 kb, respectively. A comparative analysis between the identified sheep CNVRs and those reported in cattle and goat genomes indicated that overlaps between sheep and both other species CNVRs are highly significant (P<0.0001), suggesting that several chromosome regions might contain recurrent interspecies CNVRs. Many sheep CNVRs include genes with important biological functions. Further studies are needed to evaluate their functional relevance.

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Keywords

Bagnolese
 
biological functions
 
bovine genome
 
CNVRs
 
copy number variations
 
cross species cattle-sheep array comparative genome hybridization experiment
 
functional relevance
 
goat genomes
 
identified sheep CNVRs
 
median equal
 
recurrent interspecies CNVRs
 
sheep CNVRs
 
sheep genome analysing ewes
 
species CNVRs
 
tiling oligonucleotide array
 
Valle del Belice
 
virtual sheep genome