Article

Frequency of HLA-G exon 8 polymorphisms and kidney allograft outcome in Iranian population.

Organ Transplant Research Center, Nemazi Hospital, Shiraz University of Medical Sciences, Zand Street, 7193711351 Shiraz, Iran.
Molecular Biology Reports (impact factor: 2.93). 11/2010; 38(5):3593-7. DOI:10.1007/s11033-010-0470-y
Source: PubMed

ABSTRACT The 14-bp polymorphism in exon 8 of the HLA-G gene is associated with HLA-G mRNA stability and the patterns of alternative isoform splicing and may influence the functionality of the HLA-G molecule. HLA-G expression was related to allograft acceptance and fewer episodes of acute rejection during heart, kidney and liver-kidney transplantation. In order to determine a possible correlation between the 14-bp insertion/deletion polymorphism and kidney allograft outcome in our population, genomic DNA was isolated from 144 patients who had received isolated kidney allografts. The recipients was divided into two groups, grafts presenting features of rejection group and a non-rejection group, and compared them with a control group of 100 healthy subjects. There was no significant difference in allelic frequencies of 14-bp insertion/deletion polymorphism between normal controls and kidney transplant patients. No significant difference was found between the RG and the NRG regarding the 14-bp genotypes and alleles. Therefore, additional studies with more sample size from other populations with analysis of other HLA-G polymorphisms are necessary to define this polymorphism as a valuable clinical marker.

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    Article: Human leukocyte antigen variation among Iranian renal trans- plant recipients
    Behzad Einollahi¹, Zohreh Rostami, Mojtaba Teimoori¹
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    ABSTRACT: 1. Background he human leukocyte antigen (HLA) sys-tem is being widely used in genetic studies because it is more polymorphic than the rest of the non-DNA markers together. HLA is the most informative genetic system known to date (1).Several researchers have focused on HLA as a potential risk allele in the pathogenesis of some diseases (2-4). On the other hand, HLA polymorphism is kind of epidemiological risk factor for some diseases. In addition, it is clear that the HLA complex will have to be increas-T ABSTRACT Background: HLA typing analysis is important in renal transplant patients. Objectives: We made a plan to determine the most frequent HLA antigens in Ira-nian kidney transplant patients. Patients and Methods: In a retrospective cross sectional study, HLA patterns were defined in 512 kidney transplant recipients (67% male and 33% female) from different transplant centers of Tehran, Iran between 2008 and 2011 by microcy-totoxicity assay. Results: The studies samples were of different ethnic groups of the Iranian kidney transplants. Considerable variations were observed in each HLA sub class. A2, A1, A3, A24 and A26 were the most frequent HLA-A antigens. Among HLA-B, the predominant antigens were B35; B13, B15, B13 and B18. The most frequent HLA-DR antigens were DR 4, DR11, DR1, DR3 and DR15. DQ1 showed the highest frequency and followed by DQ3 and DQ2. Conclusions: These results showed considerable heterogeneity in both HLA class I and class II antigens, which reflects recent admixture of this group with neigh-boring Middle East populations. Implication for health policy/practice/research/medical education: In a retrospective cross sectional study, HLA typing was defined in 512 kidney transplant recipients, 67% of them were male, and 33% were female, from different transplant center of Tehran, Iran between 2008 and 2011. The results suggest that both class I and class II polymorphisms of the study subjects depict consider-able heterogeneity, which reflects recent admixture of this group with neighboring middle east populations.
    Journal of Nephropathology. 11/2012; 1(1):164-169.
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Keywords

100 healthy subjects
 
14-bp insertion/deletion polymorphism
 
acute rejection
 
additional studies
 
allograft acceptance
 
alternative isoform splicing
 
control group
 
exon 8
 
HLA-G gene
 
HLA-G molecule
 
HLA-G mRNA stability
 
kidney allograft outcome
 
liver-kidney transplantation
 
non-rejection group
 
normal controls
 
NRG
 
populations
 
rejection group
 
sample size
 
valuable clinical marker