A novel cold-regulated gene, COR25, of Brassica napus is involved in plant response and tolerance to cold stress

Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, HuaZhong Normal University, Wuhan 430079, China.
Plant Cell Reports (Impact Factor: 3.07). 11/2010; 30(4):463-71. DOI: 10.1007/s00299-010-0952-3
Source: PubMed


Cold stress, which causes dehydration damage to the plant cell, is one of the most common abiotic stresses that adversely affect plant growth and crop productivity. To improve its cold-tolerance, plants often enhance expression of some cold-related genes. In this study, a cold-regulated gene encoding 25 KDa of protein was isolated from Brassica napus cDNA library using a macroarray analysis, and is consequently designated as BnCOR25. RT-PCR analysis demonstrated that BnCOR25 was expressed at high levels in hypocotyls, cotyledons, stems, and flowers, but its mRNA was found at low levels in roots and leaves. Northern blot analysis revealed that BnCOR25 transcripts were significantly induced by cold and osmotic stress treatment. The data also showed that BnCOR25 gene expression is mediated by ABA-dependent pathway. Overexpression of BnCOR25 in yeast (Schizosaccharomyces pombe) significantly enhanced the cell survival probability under cold stress, and overexpression of BnCOR25 in Arabidopsis enhances plant tolerance to cold stress. These results suggested that the BnCOR25 gene may play an important role in conferring freezing/cold tolerance in plants.

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    • " Toyobo , Osaka , Japan ) was applied in the detection system . Real - time PCR reaction was performed using the Real - time PCR Master Mix ( Toyobo ) according to the manual . The Actin gene was used as an internal control to normalize the data . Relative quantity of the target gene expression level was performed using the comparative Ct method ( Chen et al . , 2011 ) . For crude anti - oxidative enzyme extraction , 0 . 3 g of fresh leaves were ground to a fine powder in liquid nitrogen and then mixed with 4 mL sodium phosphate buffer ( 150 mM , pH 7 . 0 ) and treated with pre - cooling at 4 °C . The homogenate was transferred into a 10 - mL centrifuge tube and then centrifuged for 20 min with 13 ,"
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    • "In plant cells, extracellular freezing damages membrane systems . This damage is largely due to the acute dehydration associated with freezing (Mahajan and Tuteja 2005; Chen et al. 2011). Reactive oxygen species (ROS) that are produced in response to cold stress may contribute to membrane damage. "
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    • "For example, expression of CbCOR15 from C. bungeana was detected only in leaves after 1 day of treatment at 2 • C, but not in roots (Si et al., 2009). BnCOR25 from B. napus transcription level significantly increased in roots after 3 h cold treatment at 4 • C, and declined to low level after 6 h cold treatment and enhanced again after 12 h cold treatment (Chen et al., 2011). Different expression patterns may indicate different roles of these COR genes in each species. "
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