Article

African 2, a clonal complex of Mycobacterium bovis epidemiologically important in East Africa.

VLA Weybridge, New Haw, Surrey KT15 3NB, United Kingdom.
Journal of bacteriology (Impact Factor: 2.69). 02/2011; 193(3):670-8. DOI: 10.1128/JB.00750-10
Source: PubMed

ABSTRACT We have identified a clonal complex of Mycobacterium bovis isolated at high frequency from cattle in Uganda, Burundi, Tanzania, and Ethiopia. We have named this related group of M. bovis strains the African 2 (Af2) clonal complex of M. bovis. Af2 strains are defined by a specific chromosomal deletion (RDAf2) and can be identified by the absence of spacers 3 to 7 in their spoligotype patterns. Deletion analysis of M. bovis isolates from Algeria, Mali, Chad, Nigeria, Cameroon, South Africa, and Mozambique did not identify any strains of the Af2 clonal complex, suggesting that this clonal complex of M. bovis is localized in East Africa. The specific spoligotype pattern of the Af2 clonal complex was rarely identified among isolates from outside Africa, and the few isolates that were found and tested were intact at the RDAf2 locus. We conclude that the Af2 clonal complex is localized to cattle in East Africa. We found that strains of the Af2 clonal complex of M. bovis have, in general, four or more copies of the insertion sequence IS6110, in contrast to the majority of M. bovis strains isolated from cattle, which are thought to carry only one or a few copies.

2 Bookmarks
 · 
294 Views
  • [Show abstract] [Hide abstract]
    ABSTRACT: BackgroundThe genus Mycobacterium contains more than 100 species, most of which are classified as non-tuberculous mycobacteria (NTM). In Zambia, the NTM are slowly becoming recognized as pathogens of major public health significance with the advent of Human Immunodeficiency Virus (HIV) and Acquired Immunodeficiency Syndrome (AIDS). This study aimed at reporting the isolated NTM and ascertains their zoonotic potential and diagnostic significance in Zambia.MethodA total of 100 sputum samples were collected from three health facilities from suspected pulmonary tuberculosis human patients. In addition, 67 lymph node tissue samples from cattle and 14 from Kafue lechwe (Kobus leche kafuensis) showing tuberculosis-like lesions were collected. The samples were appropriately decontaminated and cultured on Middlebrook 7H10 and Stone brink. The isolates were then identified accordingly using the 16S ribosomal RNA analysis method.ResultsA total of 8 NTM were isolated from human sputum, 12 from cattle and 1 from the Kafue lechwe. The identified NTM included M. intracellulae, M. abscess, M. chimaera, M. bolleti, M. fortuitum and M. stomatopae sp. Nov.ConclusionThe isolation of NTM from humans and animals at the interface in Namwala district has highlighted the clinical significance and diagnostic challenge. The epidemiological investigation of NTM in the study area is therefore recommended. This should include sampling from environmental sources such as water and soil.
    BMC Research Notes 09/2014; 7(1):622. DOI:10.1186/1756-0500-7-622
    This article is viewable in ResearchGate's enriched format
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The genus Mycobacterium consists of over 120 known species, some of which (e.g. M. bovis and M. tuberculosis) contribute extensively to the burden of infectious disease in humans and animals, whilst others are commonly found in the environment but may rarely if ever be disease-causing. This paper reviews the mycobacteria found in southern Africa, focussing on those in the M. tuberculosis complex as well as the non-tuberculous mycobacteria (NTM), identifying those found in the area and including those causing disease in humans and animals, and outlines some recent reports describing the distribution and prevalence of the disease in Africa. Difficulties in diagnosis, host preference and reaction, immunology and transmission are discussed.
    Transboundary and Emerging Diseases 11/2013; 60(s1). DOI:10.1111/tbed.12159 · 2.10 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: In sub-Saharan Africa, bovine tuberculosis (bTB) is a potential hazard for animals and humans health. The goal of this study was to improve our understanding of bTB epidemiology in Burkina Faso and especially Mycobacterium bovis transmission within and between the bovine and human populations.
    PLoS Neglected Tropical Diseases 10/2014; 8(10):e3142. DOI:10.1371/journal.pntd.0003142 · 4.49 Impact Factor

Full-text

Download
69 Downloads
Available from
Jun 3, 2014