Veterinary World, Vol.1, No.3, March 2008
Feeding of balanced ration is essential for
growth and reproduction. Breeding efficiency
appears to be correlated with concentrations of trace
elements (Kumar and Vadhve, 1984). In recent years,
the interest in nutritional significance of trace minerals
in animal reproduction has markedly increased. The
imbalance or deficiency of trace elements leads to
inactive ovaries and repeat breeding in dairy animals.
(Hidiroglou, 1979). Therefore, the present study was
aimed to relate the blood profile of these compounds
(Cu, Co, Zn, and Iron) with fertility status in Surti
Material and Methods
Twenty-four Surti buffalo cows maintained at
Buffalo Breeding Farm, Hingoli were selected for
experimental work. All the animals were examined
clinically to ascertain the status of genital organs.
On the basic of above examinations the animals were
categorized into two groups.
Fertile - 12 cyclic buffaloes exhibiting sign of
oestrus evidenced by the presence of GF/CL on the
ovary formed this group.
Infertile - 12 infertile Buffaloes, which had not
shown any sign of oestrus since last two months and
had smooth non-functional ovary, formed this group.
Blood samples were collected from the cyclic
and infertile buffaloes in sterilized vials by jugular
venipuncture. All aseptic precaution were taken prior
to collection. Serum was separated and were stored
laboratory analysis. Serum concentration of trace
oC with 1-2 drops of Merthiolate (0.01%) till
Study of some serum trace minerals in cyclic and
non-cyclic surti buffaloes
M.K. Hedaoo, K.P. Khllare, M.D. Meshram, S.K. Sahatpure and M.G. Patil
Department of Animal Reproduction and Gynaecology,
Post Graduate Institute of veterinary and animal Sciences
minerals viz. Cu, Co, Zn, and Iron were determined
by Atomic Absorption Spectrophotometer. For
statistical analysis of the data, two mean comparison
described by Snedeeor and Cochran (1967) was
Results and Discussion
The average serum Copper level of cyclic
buffalo was 1.2 + 0.44 and non-cyclic buffalo 0.36 +
0.06 PPM. Non-significant difference was recorded
in the present study. The present findings are not in
agreement with Newar et al (1999) who recorded the
average serum Copper level in anoestrous and cyclic
buffaloes as 0.60 + 0.02 and 1.08 + 0.02 PPM
respectively. They found the level significantly higher
on the day of oestrus.
Hidiroglou (1979) reported that dietary
supplementation of copper improved the reproductive
performance of cattle. Deficiency in copper was
probably due to dietary imbalance and unavailability
of copper in soil and forages.
The average serum cobalt of cyclic buffalo was
0.44 + 0.08 and non-cyclic buffalo 0.47 + 0.06 PPM.
Non-significant difference was recorded in the
Vhora et al (1995) reported that the serum level
of cobalt were significantly higher in normal cyclic
than postpartum anoestrous cows.
The few studies, which are available, are not
sufficient to reflect as to how precisely cobalt
participates in reproductive processes.
Serum ConstituentsNormal cyclic
(Mean + SE)
(Mean + SE)
1.2 + 0.44
0.44 + 0.08
5.76 + 1.22
2.39 + 0.64
0.36 + 0.06
0.47 + 0.06
4.20 + 0.51
1.01 + 0.22
Table : 1. Serum levels of important trace minerals in fertile and infertile Surti buffaloes
Veterinary World, Vol.1(3): 71-72
Veterinary World, Vol.1, No.3, March 2008
The average concentration of Zinc in cyclic
buffalo was 5.76 + 1.22 and non-cyclic buffalo 4.20 +
0.51 PPM. The Zinc level recorded in cyclic buffalo
were higher than that of in non-cyclic buffalo. Non-
significant difference was observed in the present
study. Newar et al (1999) recorded serum Zinc level
0.56 + 0.05 and 1.78 + 0.03 PPM in anoestrous and
cyclic Swamp buffaloes. The levels were found
significantly higher on the day of oestrus. The present
findings are not in agreement with these findings.
Although naturally occurring Zinc deficiency is rare
in livestock but there are numerous reports of
improvement in reproductive performance following
dietary zinc supplementation.
The average serum Iron level of cyclic buffalo
was 2.39 + 0.64 and non-cyclic buffalo 1.01 + 0.22
PPM. Iron level recorded in cyclic buffalo were higher
than that of in non-cyclic buffaloes. In the present
study a non-significant difference was recorded
statistically. The present findings are higher that of
Newar et al (1999) who recorded 1.26 + 0.04 and
2.20 + 0.09 PPM in anoestrous and cyclic swamp
buffaloes. Iron, being and integral component of Hb,
is a very important trace element. However, its
deficiency has rarely been observed in grazing
livestock due to its abundant availability in all natural
The authors thank the Dr. D.B. Patil Asstt. Prof.
Dr. PDKV, Akola, for providing the facilities to conduct
1. Hindiroglon, M ( 1979) : Trace clement
and fertility in ruminants : A review. IJDS, 62 :
2.Kumar K and Vadhv e ( 1984) 5th National
Congress in Animal Reproduction held at
Pantnagar , Indian from 27-29 Febrruary.
3. Newar et al ( 1999) : S tudies on certain
micromineral status in anoestrus and cyclic
postpartum swamp buffaloes . Ind . J. Ani. Res.,
33 ( 2) :134-136.
4. Vhora et al (1995) : Studies on blood serum
levels of certain biochemical constituents in
normal cycling and anoestrus crossbred cows.
IJAR., 16 ( 2) :85-87.
5.S nedecor G.W. and Cochran W.G.( 1967) :
Statistical Methods 6thed, Oxford and IBH
publishing Co., New Delhi.
Study of some serum trace minerals in cyclic and non-cyclic surti buffaloes
Scientific study published by “ Nature” backs-up OI E
strategy on tackling emerging diseases
Paris, 22 February - A study published by Nature (February 21st) brings scientific confirmation to the
strategies and priorities long advocated by the World Organisation for Animal Health (OIE) which, will
now pursue with even more emphasis.
For the last few years, OIE messages have been on early detection and rapid response to emerging
and re-emerging animal diseases, including zoonoses (diseases transmissible to humans) in order
to safeguard global animal and public health.
The prevention and control of emerging and re-emerging animal diseases - linked with globalisation,
climatic changes and human behaviour leading to environmental changes - can best be led by well
governed Veterinary Services. Also, solidarity by richer countries toward developing countries is
crucial to the development of the needed technical capacity of the latter and will benefit the entire