Effects of microbial loading and sporulation temperature on atmospheric plasma inactivation of Bacillus subtilis spores

Abstract

This article was published in the journal, Applied Physics Letters [© American Institute of Physics] and is also available at: http://apl.aip.org/ Current inactivation studies of Bacillus subtilis spores using atmospheric-pressure glow discharges APGD do not consider two important factors, namely microbial loading at the surface of a substrate and sporulation temperature. Yet these are known to affect significantly microbial resistance to heat and hydrogen peroxide. This letter investigates effects of microbial loading and sporulation temperature on spore resistance to APGD. It is shown that microbial loading can lead to a stacking structure as a protective shield against APGD treatment and that high sporulation temperature increases spore resistance by altering core water content and cross-linked muramic acid content of B. subtilis spores. Accepted for publication