Article

Proteomic analysis of polyketide and nonribosomal peptide biosynthesis.

Department of Chemistry and Biochemistry, University of California-San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0358, USA.
Current opinion in chemical biology (impact factor: 8.3). 11/2010; 15(1):48-56. DOI:10.1016/j.cbpa.2010.10.021 pp.48-56
Source: PubMed

ABSTRACT Polyketides and non-ribosomal peptides are in a class of natural products important both as drug sources and as dangerous toxins and virulence factors. While studies over the last two decades have provided substantial characterization of the modular synthases that produce these compounds at the genetic level, their understanding at the protein level is much less understood. New proteomic platforms called an orthogonal active site identification system (OASIS) and proteomic interrogation of secondary metabolism (PrISM) have been developed to identify and quantify natural product synthase enzymes. Reviewed here, these tools offer the means to discover and analyze modular synthetic pathways that are limited by genetic techniques, opening the tools of contemporary proteomics to natural product sciences.

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Keywords

contemporary proteomics
 
drug sources
 
genetic level
 
modular synthases
 
modular synthetic pathways
 
natural product synthase enzymes
 
non-ribosomal peptides
 
orthogonal active site identification system
 
PrISM
 
protein level
 
substantial characterization
 
virulence factors
 

Jordan L Meier