New stationary phases for enrichment and separation in the 'omics' era.

Abstract

'Omics' is a general term for a broad discipline of science and engineering concerned with analyzing the interactions of biological molecular components in various 'omes'. These include genome, proteome, metabolome, expressome and interactome. 'Ome' and 'omics' are very convenient handles for describing the holistic approach for looking at complex systems. 'Omics' will not only have an impact on our understanding of biological processes, but also on the prospect of more accurately diagnosing and treating disease. The development of these 'omics' has depended on, and has also driven, advances in chromatography and electrophoresis, as well as highly sensitive and specific analytical techniques to permit the handling of large numbers of samples with high selectivity and sensitivity. The development and design of novel stationary phases for selective enrichment and separation is one of the key points for establishing a successfully running 'omics' platform. Therefore, this review demonstrates the application of different new materials developed in our laboratory, such as chromatographic stationary phases for selective and sensitive high-speed purification, enrichment and separation in genomics, proteomics and metabolomics.