Pol II waiting in the starting gates: Regulating the transition from transcription initiation into productive elongation

Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA.
Biochimica et Biophysica Acta (Impact Factor: 4.66). 11/2010; 1809(1):34-45. DOI: 10.1016/j.bbagrm.2010.11.001
Source: PubMed


Proper regulation of gene expression is essential for the differentiation, development and survival of all cells and organisms. Recent work demonstrates that transcription of many genes, including key developmental and stimulus-responsive genes, is regulated after the initiation step, by pausing of RNA polymerase II during elongation through the promoter-proximal region. Thus, there is great interest in better understanding the events that follow transcription initiation and the ways in which the efficiency of early elongation can be modulated to impact expression of these highly regulated genes. Here we describe our current understanding of the steps involved in the transition from an unstable initially transcribing complex into a highly stable and processive elongation complex. We also discuss the interplay between factors that affect early transcript elongation and the potential physiological consequences for genes that are regulated through transcriptional pausing.

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Available from: Sergei Nechaev, Aug 10, 2014
    • "There is some sequence similarity between the polymerases and some of the accessory factors between all domains (Bartlett et al. 2000; Bell and Jackson 2001), but others have evolved independently. The transition for initiation to elongation can also be a point of control (Nechaev and Adelman 2011) as can termination . RNA polymerase can bind to a promoter and then 'stall' (Core et al. 2008; Muse et al. 2007; Nechaev et al. 2010) through several, different mechanisms (FitzGerald et al. 2006; Hendrix et al. 2008; Li and Gilmour 2013). "
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