Papillary thyroid carcinoma shows elevated levels of 2-hydroxyglutarate

Department of Pathology, Children's Medical Center, Dallas, TX, USA.
Tumor Biology (Impact Factor: 3.61). 11/2010; 32(2):325-33. DOI: 10.1007/s13277-010-0125-6
Source: PubMed


Elevated levels of D: -2-hydroxyglutarate (D: -2-HG) occur in gliomas and myeloid leukemias associated with mutations of IDH1 and IDH2. L: -2-Hydroxyglutaric aciduria, an inherited metabolic disorder, predisposes to brain tumors. Therefore, we asked whether sporadic cancers, without IDH1 or IDH2 hot-spot mutations, show elevated 2-hydroxyglutarate levels. We retrieved 15 pairs of frozen papillary thyroid carcinoma (PTC) and adjacent non-neoplastic thyroid, and 14 pairs of hyperplastic nodule (HN) and adjacent non-hyperplastic thyroid. In all lesions, exon 4 sequencing confirmed the absence of known mutations of IDH1 and IDH2. We measured 2-hydroxyglutarate by liquid chromatography-tandem mass spectrometry. Compared to normal thyroid, PTCs had significantly higher D: -2-HG and L: -2-hydroxyglutarate (L: -2-HG) levels, and compared to HNs, PTCs had significantly higher D: -2-HG levels. D: -2-HG/L: -2-HG levels were not significantly different between HNs and normal thyroid. Further studies should clarify if elevated 2-hydroxyglutarate in PTC may be useful as cancer biomarker and evaluate the role of 2-hydroxyglutarate in cancer biology.

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Available from: Richard L. Boriack, Jun 19, 2015
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    • "Quantitation of ( L ) - 2HG and ( D ) - 2HG Measurement of D - 2HG and L - 2HG was performed as described previously ( Rakheja et al . , 2011a , 2011b ) . Briefly , the metabolite extracts were derivatiza - tion with ( + ) - Di - O - acetyl - L - tartaric anhydride and injected for chromato - graphic separation on an Agilent Hypersil ODS 4 . 0 3 250 mm , 5 mm column followed by detection and measurement using an API 3000 triple - quadrupole mass spectrometer equipped with an E"
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