Comparison of an expanded ataxia interactome with patient medical records reveals a relationship between macular degeneration and ataxia

Department of Cellular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.
Human Molecular Genetics (Impact Factor: 6.39). 11/2010; 20(3):510-27. DOI: 10.1093/hmg/ddq496
Source: PubMed


Spinocerebellar ataxias 6 and 7 (SCA6 and SCA7) are neurodegenerative disorders caused by expansion of CAG repeats encoding polyglutamine (polyQ) tracts in CACNA1A, the alpha1A subunit of the P/Q-type calcium channel, and ataxin-7 (ATXN7), a component of a chromatin-remodeling complex, respectively. We hypothesized that finding new protein partners for ATXN7 and CACNA1A would provide insight into the biology of their respective diseases and their relationship to other ataxia-causing proteins. We identified 118 protein interactions for CACNA1A and ATXN7 linking them to other ataxia-causing proteins and the ataxia network. To begin to understand the biological relevance of these protein interactions within the ataxia network, we used OMIM to identify diseases associated with the expanded ataxia network. We then used Medicare patient records to determine if any of these diseases co-occur with hereditary ataxia. We found that patients with ataxia are at 3.03-fold greater risk of these diseases than Medicare patients overall. One of the diseases comorbid with ataxia is macular degeneration (MD). The ataxia network is significantly (P= 7.37 × 10(-5)) enriched for proteins that interact with known MD-causing proteins, forming a MD subnetwork. We found that at least two of the proteins in the MD subnetwork have altered expression in the retina of Ataxin-7(266Q/+) mice suggesting an in vivo functional relationship with ATXN7. Together these data reveal novel protein interactions and suggest potential pathways that can contribute to the pathophysiology of ataxia, MD, and diseases comorbid with ataxia.

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    PLoS ONE 04/2014; 9(4):e95362. DOI:10.1371/journal.pone.0095362 · 3.23 Impact Factor
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    • "As compared to approaches where baits are screened against full-length prey proteins, a domain-based approach offers several advantages including the possibility of narrowing down interaction domains and of reducing the false negative discovery rate by allowing a more efficient folding of domains [32]. A similar approach used in the field of ataxia identified interactions that were missed in a previous full-length protein-based approach [33]. Interestingly, this domain-based approach enabled us to analyze possible bias in interacting domains. "
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