Transcriptional profiling of antimicrobial peptides avian β-defensins in the chicken ovary during sexual maturation and in response to Salmonella Enteritidis infection

Department of Animal Production, Aristotle University of Thessaloniki, Thessaloniki,
Research in Veterinary Science (Impact Factor: 1.51). 11/2010; 92(1):60-5. DOI: 10.1016/j.rvsc.2010.10.010
Source: PubMed

ABSTRACT Avian β-defensins (AvβDs) are antimicrobial peptides that play significant roles in the innate immune system in chickens. The aim of this study was to identify the types of AvβDs expressed in the chicken ovary, to investigate the effects of sexual maturation in the ovarian mRNA abundance and to determine the changes in their expression levels as a result to Salmonella enteritidis (SE) infection. RNA was extracted from the ovary of healthy prepubertal, sexually mature and aged birds, as well as from sexually mature and aged SE infected birds. Real-time PCR analysis revealed that 11 AvβDs genes were expressed in the chicken ovary. A significant up regulation of AvβD1, 3, 4, 5, 7 and 11 was observed in the ovary of sexually mature and aged birds. Furthermore, a significant up-regulation of AvβD4, 5, 7, 11 and 12 was observed in the ovary of SE infected sexually mature birds. These results suggest that the mRNA expression of at least six AvβDs increase with age in the ovary of laying hens, and that at least five AvβDs show an induction in their expression in response to SE infection, indicating an AvβD-mediated immune response mechanism in the chicken ovary.

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    • "Scale bars represent 20 µm. (Abdelsalam et al., 2010) increased expression of several AvBDs (Michailidis et al., 2012). Injection of birds with LPS resulted in an increase in the expression of several AvBDs in the theca tissue, although no response was observed in the granulosa layer (Subedi et al., 2007). "
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