Article

Production, purification and characterization of adeno-associated vectors.

Center of Animal Biotechnology and Gene Therapy and Department of Biochemistry and Molecular Biology, School of Veterinary Medicine, Universitat Autònoma de Barcelona, 08193-Bellaterra, Spain.
Current Gene Therapy (impact factor: 3.39). 11/2010; 10(6):423-36. pp.423-36
Source: PubMed

ABSTRACT The use of recombinant adeno-associated viral vectors (rAAVs) as gene transfer tools has increased dramatically during the past last several years, establishing AAV as the vector of choice for many therapeutic applications. With the steady advance of the field toward clinical studies, and the isolation and engineering of several novel AAV serotypes, efficient, scalable, and versatile production and purification methods are continuously under development. Here, we review the current state of the art in the various production and purification methods for rAAVs. Classical parameters and methodologies to characterize rAAV stocks will be also discussed.

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Keywords

AAV
 
Classical parameters
 
clinical studies
 
current state
 
gene transfer tools
 
novel AAV serotypes
 
purification methods
 
rAAV stocks
 
rAAVs
 
recombinant adeno-associated viral vectors
 
scalable
 
steady advance
 
therapeutic applications
 
various production
 
vector
 
versatile production
 

Eduard Ayuso