Article

Hyperbranched acidic polysaccharide from green tea.

Institute of Polymer Science, School of Chemistry and Chemical Engineering, BME Center, State Key Laboratory of Optoelectronic Materials and Technologies, DSAPM Lab and PCFM Lab, Sun Yat-sen University, Guangzhou 510275, China.
Biomacromolecules (impact factor: 5.48). 10/2010; 11(12):3395-405. DOI:10.1021/bm100902d pp.3395-405
Source: PubMed

ABSTRACT An acidic tea polysaccharide (ALTPS), isolated from green tea ( Camellia sinensis ), was characterized as a hyperbranched glycoprotein containing the acidic heteropolysaccharide chains and the protein residues from the results of UV-vis, FTIR, one- and two-dimensional NMR, GC, GC-MS, and amino acid analyses. Solution properties of ALTPS were investigated by static and dynamic light scattering analyses and viscometry. The results indicated that the viscosity behavior of ALTPS exhibited a typical polyelectrolyte effect in distilled water, which may be avoided by adding salts. The low intrinsic viscosity of ALTPS in the solutions (8-15 mL/g) is attributed to its hyperbranched structure. By application of the polymer solution theory, it was revealed that ALTPS was present in a sphere-like conformation in the solutions as a result of the hyperbranched structure. The TEM image further confirmed that ALTPS existed in a spherical conformation in aqueous NaCl solution. Glucose was absorbed by ALTPS, which may be one of blood glucose lowering mechanisms of tea polysaccharides.

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Keywords

ALTPS
 
ALTPS exhibited
 
amino acid analyses
 
aqueous NaCl solution
 
blood glucose
 
Camellia sinensis
 
dynamic light scattering analyses
 
Glucose
 
hyperbranched glycoprotein
 
low intrinsic viscosity
 
protein residues
 
Solution properties
 
solutions
 
sphere-like conformation
 
tea polysaccharides
 
TEM image
 
typical polyelectrolyte effect
 
viscosity behavior