Article

[Study of HBV-DNA in ovarian granulosa cells of hepatitis B carriers].

Reproductive Research Center, Peking University Third Hospital, Beijing 100191, China.
Zhonghua yi xue za zhi 07/2010; 90(25):1778-80. pp.1778-80
Source: PubMed

ABSTRACT To study the infection status of hepatitis B virus in ovarian granulosa cells of female hepatitis B carriers.
The follicular fluid of 6 female carriers of hepatitis B (HBsAg+, HBeAg+, HBcAb+) was used to extract granule cells and prepare smears. The samples were detected via in situ hybridization by digoxin-labeled HBV-DNA probe. The color reaction system of alkaline phosphates was used. And the positive hybridization signal was purple blue.
Three granulosa cell samples, located at the nuclei of granulosa cells, were HBV-DNA positive.
HBV DNA exists in the granulosa cells of hepatitis B patients. Thus HBV infection may be vertically transmitted to the offspring via the infected reproductive cells.

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Keywords

6 female carriers
 
alkaline phosphates
 
color reaction system
 
digoxin-labeled HBV-DNA probe
 
female hepatitis B carriers
 
follicular fluid
 
granulosa cell samples
 
granulosa cells
 
HBcAb+
 
HBsAg+
 
HBV DNA
 
HBV infection
 
HBV-DNA positive
 
infected reproductive cells
 
offspring
 
ovarian granulosa cells