[Study of HBV-DNA in ovarian granulosa cells of hepatitis B carriers].
ABSTRACT To study the infection status of hepatitis B virus in ovarian granulosa cells of female hepatitis B carriers.
The follicular fluid of 6 female carriers of hepatitis B (HBsAg+, HBeAg+, HBcAb+) was used to extract granule cells and prepare smears. The samples were detected via in situ hybridization by digoxin-labeled HBV-DNA probe. The color reaction system of alkaline phosphates was used. And the positive hybridization signal was purple blue.
Three granulosa cell samples, located at the nuclei of granulosa cells, were HBV-DNA positive.
HBV DNA exists in the granulosa cells of hepatitis B patients. Thus HBV infection may be vertically transmitted to the offspring via the infected reproductive cells.