Article

Basophil CD203c Levels Are Increased at Baseline and Can Be Used to Monitor Omalizumab Treatment in Subjects with Nut Allergy

Department of Pediatrics, Stanford University School of Medicine, Stanford, CA 94305, USA.
International Archives of Allergy and Immunology (Impact Factor: 2.43). 10/2010; 154(4):318-27. DOI: 10.1159/000321824
Source: PubMed

ABSTRACT Basophils contribute to anaphylaxis and allergies. We examined the utility of assessing basophil-associated surface antigens (CD11b/CD63/CD123/CD203c/CD294) in characterizing and monitoring subjects with nut allergy.
We used flow cytometry to analyze basophils at baseline (without any activation) and after ex vivo stimulation of whole blood by addition of nut or other allergens for 2, 10, and 30 min. We also evaluated whether basophil expression of CD11b/CD63/CD123/CD203c/CD294 was altered in subjects treated with anti-IgE monoclonal antibody (omalizumab) to reduce plasma levels of IgE.
We demonstrate that basophil CD203c levels are increased at baseline in subjects with nut allergy compared to healthy controls (13 subjects in each group, p < 0.0001). Furthermore, we confirm that significantly increased expression of CD203c occurs on subject basophils when stimulated with the allergen to which the subject is sensitive and can be detected rapidly (10 min of stimulation, n = 11, p < 0.0008). In 5 subjects with severe peanut allergy, basophil CD203c expression following stimulation with peanut allergen was significantly decreased (p < 0.05) after 4 and 8 weeks of omalizumab treatment but returned toward pretreatment levels after treatment cessation.
Subjects with nut allergy show an increase of basophil CD203c levels at baseline and following rapid ex vivo stimulation with nut allergen. Both can be reduced by omalizumab therapy. These results highlight the potential of using basophil CD203c levels for baseline diagnosis and therapeutic monitoring in subjects with nut allergy.

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    • "sting levels of CD203c are also reportedly high on baso - phils analyzed from food allergic subjects , suggesting an on - going activation of these cells in vivo . Interestingly , both omalizumab administration ( or anti - IgE therapy ) and sublin - gual / oral immunotherapy ( or desensitization ) protocols are reported to reduce this expression ( Gernez et al . , 2011 ; Keet et al . , in press ) . Decreased allergen - induced expression of CD63 has also been seen in the setting of many immunomodulatory - based trials . However , the specificity of these markers with respect to basophil degranulation has been questioned , and additional concerns relating to the ki - netics of expression , the addition"
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